Pattern recognition analysis of proton nuclear magnetic resonance spectra of postmortem cerebrospinal fluid from rats with drug-induced seizure or coma
Pattern recognition analysis of proton nuclear magnetic resonance spectra of postmortem cerebrospinal fluid from rats with drug-induced seizure or coma
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DOI：
10.1016/j.legalmed.2017.01.004
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发表时间：
2017-03-01
期刊：
影响因子：
1.5
通讯作者：
Ohno, Youkichi
中科院分区：
文献类型：
--
作者：
Kanawaku, Yoshimasa;Hirakawa, Keiko;Ohno, Youkichi
脑脊液（CSF）通常在尸检调查中进行总体评估；然而，其在化学评价中的应用尚未完全实现。采用模式识别方法对核磁共振 (NMR) 谱进行分析，应用于脑脊液样本。大鼠用戊四氮（PTZ）诱导癫痫发作，或用戊巴比妥（PB）诱导昏迷，CO2气体安乐死后收集死后脑脊液。对个体死后脑脊液样本进行核磁共振数据的模式识别分析。本研究的目的是确定 NMR 数据的模式识别分析是否可用于根据药物治疗对大鼠进行分类。还评估了使用死后脑脊液进行模式识别分析的 NMR 数据的适用性。偏最小二乘判别分析 (PLS-DA) 评分图表明 PTZ、PB 和 NS（对照组）组聚集且清晰分离。 PLS-DA 相关载荷图显示，因子 1 的光谱和类别方差分别为 41% 和 42%，因子 2 的光谱和类别方差分别为 17% 和 27%。因此，因子 1 和 2 总共分别描述了 58% (41% + 17%) 和 69% (42% + 27%) 的变异。死后脑脊液的核磁共振研究有可能被用作一种新型法医神经化学方法和临床环境。 (C) 2017 Elsevier B.V. 保留所有权利。
Cerebrospinal fluid (CSF) is routinely subjected to gross evaluation in postmortem investigations; however, its use in chemical evaluations has not been fully realized. Analysis of nuclear magnetic resonance (NMR) spectra with pattern recognition methods was applied to CSF samples. Rats were treated with pentylenetetrazol (PTZ) to induce seizure or pentobarbital (PB) to induce coma, and postmortem CSF was collected after CO2 gas euthanization. Pattern recognition analysis of the NMR data was performed on individual postmortem CSF samples. The aim of this study was to determine if pattern recognition analysis of NMR data could be used to classify the rats according to their drug treatment. The applicability of NMR data with pattern recognition analysis using postmortem CSF was also assessed. Partial Least Squares-Discriminant Analysis (PLS-DA) score plots indicated that the PTZ, PB, and NS (control) groups were clustered and clearly separated. PLS-DA correlation loading plots showed respective spectral and category variances of 41% and 42% for factor 1, and 17% and 27% for factor 2. Thus, factors 1 and 2 together described 58% (41% + 17%) and 69% (42% + 27%) of the variation, respectively. NMR study of postmortem CSF has the potential to be utilized as both a novel forensic neurochemistry method and in the clinical setting. (C) 2017 Elsevier B.V. All rights reserved.