IN-VITRO BINDING OF THE 2-FINGER REPRESSOR CREA TO SEVERAL CONSENSUS AND NONCONSENSUS SITES AT THE IPNA UPSTREAM REGION IS CONTEXT-DEPENDENT
IN-VITRO BINDING OF THE 2-FINGER REPRESSOR CREA TO SEVERAL CONSENSUS AND NONCONSENSUS SITES AT THE IPNA UPSTREAM REGION IS CONTEXT-DEPENDENT
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DOI：
10.1016/0014-5793(94)80581-4
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发表时间：
1994-03-28
期刊：
影响因子：
3.5
通讯作者：
PENALVA, MA
中科院分区：
文献类型：
--
作者：
ESPESO, EA;PENALVA, MA
构巢曲霉阻遏物CreA的两个锌指识别共有六核苷酸5 '-SYGGRG-3'。我们已经确定了所有的CreA结合位点在一个类似的ipnA基因上游2 kb的区域。我们的分析表明，（i）CreA结合到某些共识网站在上下文依赖性的方式;（ii）五个非共识6 bp序列也被CreA识别;这种非典型的结合与第二个相邻的CreA结合位点的存在相关，这表明，识别两个连接的网站稳定CreA结合。我们的研究结果表明，CreA的结合可能性可能比最初设想的更复杂。
The two zinc-fingers of the Aspergillus nidulans repressor CreA recognize the consensus hexanucleotide 5'-SYGGRG-3'. We have determined all the CreA binding sites in a similar to 2 kb region upstream the ipnA gene. Our analysis shows that (i) CreA binds to certain consensus sites in a context-dependent manner; (ii) five non-consensus 6-bp sequences are also recognized by CreA; this non-canonical binding correlates with the presence of a second, neighbouring CreA binding site, suggesting that recognition of two linked sites stabilizes CreA binding. Our results suggest that the binding possibilities of CreA might be more complex than originally envisaged.