3D Printed Bioceramic Scaffolds as a Universal Therapeutic Platform for Synergistic Therapy of Osteosarcoma
3D Printed Bioceramic Scaffolds as a Universal Therapeutic Platform for Synergistic Therapy of Osteosarcoma
复制标题
3D 打印生物陶瓷支架作为骨肉瘤协同治疗的通用治疗平台
DOI：
10.1021/acsami.1c00553
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发表时间：
2021-04-15
影响因子：
9.5
通讯作者：
Li, Mingqiang
中科院分区：
文献类型：
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作者：
Dang, Wentao;Yi, Ke;Li, Mingqiang
临床骨肉瘤治疗中肿瘤复发和化疗耐药性的存在，迫切需要开发一种局部植入物，既能选择性杀伤残留的肿瘤细胞，又能有效填充肿瘤切除后骨缺损。本文通过在磷酸三钙（TCP）支架表面连续涂覆TiN微粒和阿霉素（DOX），开发了一种多功能平台，以实现骨肉瘤光热治疗和化疗的协同效应。通过将支架浸泡在含有不同浓度TiN和DOX的溶液中，可以灵活调节支架中TiN和DOX的含量。通过精确的光热治疗和局部控释化疗，在体内外均取得了良好的治疗效果。此外，当将支架植入手术切除骨肉瘤所导致的骨缺损中时，整体散装支架为骨组织提供机械支撑。重要的是，使用聚（D，L-丙交酯）（PDLLA）作为介质，支架可以被开发作为一个通用的平台，用于负载不同种类的治疗药物。这项研究可能为设计多功能局部植入物提供见解，用于在手术干预后根除肿瘤，减轻副作用。
The postoperative tumor recurrence and chemotherapy resistance in clinical osteosarcoma treatment have raised an imperative need to develop local implants for selectively killing residual tumor cells and simultaneously provide a scaffold for effectively filling the tumor resection-induced bone defects. Herein, a multifunctional platform is developed through successively coating TiN microparticles and doxorubicin (DOX) on the surface of tricalcium phosphate (TCP) scaffolds to achieve synergetic effects of photothermal therapy and chemotherapy for osteosarcoma. The content of TiN and DOX in the scaffolds can be flexibly adjusted by immersing the scaffolds into the solution containing different concentrations of TiN and DOX. The excellent therapeutic effect was achieved both in vitro and in vivo through the precise photothermal therapy and localized controlled-release chemotherapy. Moreover, the overall bulk scaffolds provide the mechanical support for bone tissue when implanting scaffolds into bone defects resulting from surgical removal of osteosarcoma. Importantly, using the poly(D,L-lactide) (PDLLA) as the medium, the scaffolds can be exploited as a universal platform for loading different kinds of therapeutic agents. This study may provide insights into designing multifunctional local implantation for eradicating tumors after surgical interventions with mitigated side effects.