Proteomic analysis of cerebrospinal fluid from multiple sclerosis patients
Proteomic analysis of cerebrospinal fluid from multiple sclerosis patients
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DOI：
10.1002/pmic.200300715
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发表时间：
2004-07-01
期刊：
影响因子：
3.4
通讯作者：
Robben, J
中科院分区：
文献类型：
--
作者：
Dumont, D;Noben, JP;Robben, J
多发性硬化症是一种中枢神经系统自身免疫性炎性脱髓鞘疾病。多发性硬化症在分子水平上的发病机制仍然知之甚少，目前还没有可靠的蛋白质类疾病标志物可用。本研究的目的是建立多发性硬化症患者脑脊液蛋白质双向凝胶电泳法分离蛋白质数据库。用液相色谱质谱联用从300个点鉴定出65个不同的蛋白质。其中18种蛋白在脑脊液2-DE凝胶上未见报道，本文报道了这些蛋白的鉴定，并讨论了它们与多发性硬化症的潜在关系。
Multiple sclerosis is an autoimmune inflammatory demyelinating disease of the central nervous system. Disease mechanisms in multiple sclerosis at the molecular level remain poorly understood and no reliable proteinaceous disease markers are available yet. The goal of the present study is the construction of a protein database of two-dimensional gel electrophoresis (2-DE) separated cerebrospinal fluid (CSF) proteins from multiple sclerosis patients. By means of liquid chromatography tandem mass spectrometry 65 different proteins were identified from 300 spots. Eighteen of these proteins have not been reported previously on 2-DE gels of CSF Here we report on the identification of these proteins and discuss their potential relation to multiple sclerosis.