Eradication of Propionibacterium acnes by its endogenic porphyrins after illumination with high intensity blue light
Eradication of Propionibacterium acnes by its endogenic porphyrins after illumination with high intensity blue light
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DOI：
10.1016/s0928-8244(02)00423-6
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发表时间：
2003-01-21
影响因子：
--
通讯作者：
Nitzan, Y
中科院分区：
文献类型：
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作者：
Ashkenazi, H;Malik, Z;Nitzan, Y
痤疮丙酸杆菌是一种革兰氏阳性、微需氧细菌，可引起皮肤伤口。已知其天然产生大量的细胞内卟啉。本研究的结果证实了所研究的痤疮丙酸杆菌菌株能够在不需要任何触发分子的情况下产生内源性卟啉。来自生长培养物的提取物在405 nm处激发时显示出约612 nm的发射峰，这是卟啉的特征。内源性卟啉测定和定量后，从细菌细胞中提取的荧光强度和洗脱保留时间的高效液相色谱法（HPLC）。由痤疮丙酸杆菌产生的卟啉主要是粪卟啉，如HPLC洗脱模式所示。加入δ-氨基乙酰丙酸（ALA）可促进胞内卟啉的合成，并产生更多的粪卟啉。用407 - 420 nm的蓝光照射痤疮丙酸杆菌，观察其内源卟啉对痤疮丙酸杆菌的清除作用。当用75 J cm（-2）的光剂量照射一次时，在液体培养基中厌氧生长24 h的培养物的活力降低不到两个数量级。以75 J·cm~（-2）的光剂量连续照射2~3次，间隔24 h，可获得较好的光动力学效应。在这些条件下的文化的生存能力下降了四个数量级后，两个照明和五个数量级后，三个照明。当ALA触发的培养物用751 cm-2的光剂量的强烈蓝光照射时，处理过的培养物的活力降低了7个数量级。即使在指示光强度的单次照射后，也可能发生活力的这种降低。X射线显微分析和透射电子显微镜显示，在照明痤疮丙酸杆菌膜结构损伤。用蓝光（407 - 420 nm）照射内源粪卟啉显然在痤疮丙酸杆菌光灭活中起主要作用。具有一系列若干照射或在应用ALA后照射的治疗方案可适用于治愈痤疮。通过两种途径的治疗可以克服痤疮丙酸杆菌对抗生素治疗的抗性。(C)2002年，欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
Propionibacterium acnes is a Gram-positive, microaerophilic bacterium that causes skin wounds. It is known to naturally produce high amounts of intracellular porphyrins. The results of the present study confirm that the investigated strain of P. acnes is capable of producing endogenic porphyrins with no need for any trigger molecules. Extracts from growing cultures have demonstrated emission peaks around 612 nm when excited at 405 nm, which are characteristic for porphyrins. Endogenic porphyrins were determined and quantified after their extraction from the bacterial cells by fluorescence intensity and by elution retention time on high-performance liquid chromatography (HPLC). The porphyrins produced by P. acnes are mostly coproporphyrin, as shown by the HPLC elution patterns. Addition of delta-aminolevulinic acid (ALA) enhanced intracellular porphyrin synthesis and higher amounts of coproporphyrin have been found. Eradication of P. acnes by its endogenic porphyrins was examined after illumination with intense blue light at 407-420 nm. The viability of 24 h cultures grown anaerobically in liquid medium was reduced by less than two orders of magnitude when illuminated once with a light dose of 75 J cm(-2). Better photodynamic effects were obtained when cultures were illuminated twice or three times consecutively with a light dose of 75 J cm(-2) and an interval of 24 h between illuminations. The viability of the culture under these conditions decreased by four orders of magnitude after two illuminations and by five orders of magnitude after three illuminations. When ALA-triggered cultures were illuminated with intense blue light at a light dose of 751 cm(-2) the viability of the treated cultures decreased by seven orders of magnitude. This decrease in viability can occur even after a single exposure of illumination for the indicated light intensity. X-ray microanalysis and transmission electron microscopy revealed structural damages to membranes in the illuminated P. acnes. Illumination of the endogenous coproporphyrin with blue light (407-420 nm) apparently plays a major role in P. acnes photoinactivation. A treatment protocol with a series of several illuminations or illumination after application of ALA may be suitable for curing acne. Treatment by both pathways may overcome the resistance of P. acnes to antibiotic treatment. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.