PHENOL AND BENZOATE METABOLISM BY PSEUDOMONAS PUTIDA - REGULATION OF TANGENTIAL PATHWAYS
PHENOL AND BENZOATE METABOLISM BY PSEUDOMONAS PUTIDA - REGULATION OF TANGENTIAL PATHWAYS
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DOI：
10.1128/jb.100.2.869-877.1969
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发表时间：
1969-01-01
影响因子：
3.2
通讯作者：
HEGEMAN, GD
中科院分区：
文献类型：
--
作者：
FEIST, CF;HEGEMAN, GD
儿茶酚是恶臭假单胞菌菌株代谢苯甲酸盐和苯酚的中间体。在以苯甲酸盐为代价的生长过程中，儿茶酚被裂解（1,2-加氧酶）并通过 β-酮己二酸途径代谢；在以苯酚或甲酚为代价的生长过程中，所形成的儿茶酚或取代的儿茶酚在儿茶酚的芳环的间(2,3-加氧酶)裂解后通过单独的途径代谢。可以解释在苯酚和苯甲酸盐生长的P细胞中间位和正向途径酶的相互排斥的发生。 putidaon 的基础是这两种途径的调节模式的差异。通过使用不可代谢诱导剂和阻断突变体，获得了一些元途径酶的无偿合成。元途径的所有四种酶均由主要底物甲酚或苯酚或其类似物诱导。催化儿茶酚转化为β-酮己二酸烯醇内酯的正向途径的三种酶是由顺式，顺式粘康酸诱导的，顺式，顺式粘康酸是通过1,2-加氧酶介导的裂解从儿茶酚产生的。对两条途径的诱导特异性和功能差异的观察表明，它们实际上并不是相切的或多余的。元途径是衍生自取代酚类化合物的儿茶酚的各种烷基衍生物分解代谢的一般机制。正向途径更具特异性，主要参与儿茶酚前体和儿茶酚本身的分解代谢。
Catechol occurs as an intermediate in the metabolism of both benzoate and phenol by strains ofPseudomonas putida. During growth at the expense of benzoate, catechol is cleavedortho(1,2-oxygenase) and metabolized via the β-ketoadipate pathway; during growth at the expense of phenol or cresols, the catechol or substituted catechols formed are metabolized by a separate pathway followingmeta(2,3-oxygenase) cleavage of the aromatic ring of catechol. It is possible to explain the mutually exclusive occurrence of themetaandorthopathway enzymes in phenol- and benzoate-grown cells ofP. putidaon the basis of differences in the mode of regulation of these two pathways. By use of both nonmetabolizable inducers and blocked mutants, gratuitous synthesis of some of themetapathway enzymes was obtained. All four enzymes of themetapathway are induced by the primary substrate, cresol or phenol, or its analogue. Three enzymes of theorthopathway that catalyze the conversion of catechol to β-ketoadipate enol-lactone are induced bycis,cis-muconate, produced from catechol by 1,2-oxygenase-mediated cleavage. Observations on the differences in specificity of induction and function of the two pathways suggest that they are not really either tangential or redundant. Themetapathway serves as a general mechanism for catabolism of various alkyl derivatives of catechol derived from substituted phenolic compounds. Theorthopathway is more specific and serves primarily in the catabolism of precursors of catechol and catechol itself.