Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina.
Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina.
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DOI：
10.1038/srep42330
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发表时间：
2017-02-10
影响因子：
4.6
通讯作者：
Sernagor E
中科院分区：
文献类型：
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作者：
Hilgen G;Pirmoradian S;Pamplona D;Kornprobst P;Cessac B;Hennig MH;Sernagor E
我们研究了小鼠视网膜神经节细胞（RGC）中光驱动反应的个体发生。使用大规模的，高密度的多电极阵列，我们记录了从数百到数千个RGC同时在全视网膜水平，包括背侧和腹侧的位置。对不同对比度的反应不仅揭示了ON、OFF和ON-OFF反应的复杂发育特征，而且揭示了背侧和腹侧RGC反应的差异。在睁开眼睛，背RGC的所有类型的光反应更灵敏，也许表明环境的优先权，以巢查看断奶前的小狗。ON和OFF反应的发展概况表现出拮抗行为，最强的ON反应后不久，眼睛睁开，随后在OFF反应的强度增加later on. Further，我们发现，随着成熟的感受野（RF）中心大小的减少，尖峰触发的平均反应白色噪声变得更强，中心变得更圆，同时保持RGC类型之间的差异。我们的结论是，视网膜功能的成熟不是空间上均匀的，可能反映了生态的要求，有利于更早地成熟的背侧视网膜。
We have investigated the ontogeny of light-driven responses in mouse retinal ganglion cells (RGCs). Using a large-scale, high-density multielectrode array, we recorded from hundreds to thousands of RGCs simultaneously at pan-retinal level, including dorsal and ventral locations. Responses to different contrasts not only revealed a complex developmental profile for ON, OFF and ON-OFF responses, but also unveiled differences between dorsal and ventral RGC responses. At eye-opening, dorsal RGCs of all types were more responsive to light, perhaps indicating an environmental priority to nest viewing for pre-weaning pups. The developmental profile of ON and OFF responses exhibited antagonistic behaviour, with the strongest ON responses shortly after eye-opening, followed by an increase in the strength of OFF responses later on. Further, we found that with maturation receptive field (RF) center sizes decrease, spike-triggered averaged responses to white noise become stronger, and centers become more circular while maintaining differences between RGC types. We conclude that the maturation of retinal functionality is not spatially homogeneous, likely reflecting ecological requirements that favour earlier maturation of the dorsal retina.