Immunocytochemical in vivo localization of fibronectin-rich contact sites on fibroblasts of normal periodontal ligament and inflamed gingiva.
Immunocytochemical in vivo localization of fibronectin-rich contact sites on fibroblasts of normal periodontal ligament and inflamed gingiva.
复制标题
DOI：
10.1111/j.1600-0765.1988.tb01364.x
复制
发表时间：
1988-07
影响因子：
3.5
通讯作者：
M. Cho;P. Garant;Y. L. Lee
中科院分区：
文献类型：
--
作者：
M. Cho;P. Garant;Y. L. Lee
用常规电子显微镜和免疫细胞化学方法研究成纤维细胞与基质的附着部位。用兔抗比格犬血浆纤维连接蛋白抗体和羊抗兔抗体与辣根过氧化物酶或铁蛋白偶联，将纤维连接蛋白定位于成纤维细胞与基质的附着部位。在健康牙周膜的成纤维细胞中，附着部位由与质膜外表面并列的无定形材料组成的矩形斑块组成。在这些部位，细胞膜染色较浓，邻近细胞质的特点是密度增加，胞质细丝浓度较高。细胞外斑块中含有纤维连接蛋白。形态计量学分析表明，附着斑块厚约90 nm，宽约250 nm，长约550 nm，均匀分布于胞体和胞质突起周围。在炎症牙龈中，附着部位较大，形状不规则，细胞表面有较多的细胞外无定形物质和纤维连接蛋白。胞质纤维更多地以应力纤维的形式捆绑在一起，终止于与细胞外纤维连接蛋白包被的纤维连接类型的连接处。
Fibroblast-to-matrix attachment sites were studied by routine electron microscopy and immunocytochemistry. Rabbit antibodies to beagle dog plasma fibronectin and sheep antirabbit antibodies conjugated with horseradish peroxidase or ferritin were used to localize fibronectin at fibroblast-to-matrix attachment sites. In fibroblasts of healthy periodontal ligament, the attachment sites consisted of rectangular patches of amorphous material juxtaposed to the external surface of the plasma membrane. At these sites, the cell membrane was more densely stained and the adjacent cytoplasm was characterized by increased density and a high concentration of cytoplasmic filaments. The extracellular plaques contained fibronectin. Morphometric analysis indicated that the attachment plaques were approximately 90 nm thick, 250 nm wide, and 550 nm long, and distributed uniformly over both the cell body and peripheral cytoplasmic processes. In inflamed gingiva, the attachment sites were larger, irregular in shape, and with greater amounts of extracellular amorphous material and fibronectin associated to the cell surface. Cytoplasmic filaments were more often bundled as stress fibers which terminated in fibronexus-type junctions with extracellular fibronectin-coated filaments.