Cytotoxicity of materials used in perforation repair tested using the V79 fibroblast cell line and the granulocyte-macrophage progenitor cells
Cytotoxicity of materials used in perforation repair tested using the V79 fibroblast cell line and the granulocyte-macrophage progenitor cells
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DOI：
10.1111/j.1365-2591.2005.01045.x
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发表时间：
2006-01-01
影响因子：
5
通讯作者：
Bincoletto, C
中科院分区：
文献类型：
--
作者：
Souza, NJA;Justo, GZ;Bincoletto, C
目的比较永久性V79成纤维细胞和小鼠粒细胞-巨噬细胞祖细胞（CFU-GM）修复穿孔材料的细胞毒性。MTA和牙胶用培养基洗脱72小时，通过将提取物与V79和骨髓孵育来评估其细胞毒性。衍生的祖细胞分别培养24小时和7天。用总核酸含量（NAC）、中性红摄取（NRU）和四氮唑盐还原（MTT）法测定细胞毒性。用重组鼠粒细胞-巨噬细胞集落刺激因子刺激的克隆培养物中测定的骨髓CFU-GM集落数用于评估对祖细胞的细胞毒性。统计学分析采用单因素方差分析和Tukey检验，其中appropriate.Results所有材料的细胞毒性在两个细胞系统，但是，CFU-GM是更敏感的提取物比V79细胞。NAC法和MTT法对V79细胞的毒性大小顺序相似：玻璃离子> SuperEBA + N-Rickert>牙胶+MTA+银汞合金（P < 0.05）。相比之下，NRU试验对MTA、杜仲胶和汞合金浸提液的敏感性较低。在克隆培养试验中，在杜仲胶、SuperEBA和MTA的存在下，毒性不太明显。相似的细胞反应被发现通过直接放置在克隆culture disks.Conclusions毒性的敏感性取决于终点和细胞培养系统的选择。然而，MTA在两种细胞系统中均被评为细胞毒性最小的骨水泥。
Aim To compare the cytotoxicity of materials used to repair perforations using permanent V79 fibroblasts and murine granulocyte-macrophage progenitor cells (CFU-GM).Methodology Set specimens from amalgam, glass-ionomer, SuperEBA, N-Rickert, MTA and gutta-percha were eluted with culture medium for 72 h and their cytotoxicities were assessed by incubating the extracts with V79 and bone marrow-derived progenitors for 24 h and 7 days, respectively. Cytotoxicity on V79 cells was judged using the total nucleic acid content (NAC), neutral red uptake (NRU) and reduction of the tetrazolium salt (MTT). The number of bone marrow CFU-GM colonies determined in clonal cultures stimulated with recombinant murine granulocyte-macrophage colony-stimulating factor was used to assess cytotoxicity to progenitor cells. Statistical analyses were conducted using the one-way analysis of variance and Tukey's test where appropriate.Results All materials were cytotoxic in both cell systems; however, CFU-GM was more sensitive to the extracts than V79 cells. A similar rank order of toxicity was observed in V79 cells using the NAC and the MTT assays: glass-ionomer > N-Rickert congruent to SuperEBA > gutta-percha > amalgam congruent to MTA (P < 0.05). In contrast, the NRU test exhibited a lower sensitivity to MTA, gutta-percha and amalgam extracts. In the clonal culture assay, the toxicity was less pronounced in the presence of gutta-percha, SuperEBA and MTA. Similar cellular responses were found by placing the set specimens directly in the clonal culture dishes.Conclusions The sensitivity of toxicity depended on the choice of the endpoint and the cell-culture system. Nevertheless, MTA was ranked as the least cytotoxic cement in both cell systems.