Root filtering, rather than host identity or age, determines the composition of root-associated fungi and oomycetes in three naturally co-occurring Brassicaceae
Root filtering, rather than host identity or age, determines the composition of root-associated fungi and oomycetes in three naturally co-occurring Brassicaceae
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DOI：
10.1016/j.soilbio.2020.107806
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发表时间：
2020-07-01
影响因子：
9.7
通讯作者：
Thines, Marco
中科院分区：
文献类型：
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作者：
Macia-Vicente, Jose G.;Nam, Bora;Thines, Marco
十字花科植物包括一些研究最多的植物微生物宿主，提高了我们对微生物多样性、群落组装规则和对宿主性能影响的认识。与细菌相比，芸苔科微生物组中的真核生物研究尚不充分，特别是在野外条件下。在此，我们利用从一个长期未被破坏的地点采集的三个一年生共居芸苔科植物的两个生长阶段的根和伴生土壤的DNA元条形码，评估了寄主身份和年龄对真菌和卵菌根群落聚集的影响。我们的研究结果表明，根相关真菌的群落比卵菌的群落更具多样性和结构。在这两种情况下，植物的特性和生长阶段对真菌和卵菌的群落结构影响不大，而土壤/根隔室的过滤作用很强，导致根系中的微生物数量相对于周围土壤减少。土壤中特定真菌和卵菌（如Helotiales或Peronosporales成员）在根中的富集表明，植物组织的无症状定植可能与寄主的健康和健康有关。
The plant family Brassicaceae includes some of the most studied hosts of plant microbiomes, improving our knowledge of microbial diversity, community assembly rules, and effects on host performance. Compared to bacteria, eukaryotes in the microbiome of Brassicaceae remain understudied, especially under field conditions. Here, we assessed the impact of host identity and age on the assembly of fungal and oomycete root communities, using DNA metabarcoding of roots and associated soil of three annual co-habiting Brassicaceae collected at two growth stages from a site untouched for a long time. Our results showed that communities of root-associated fungi are more diverse and structured across factors than those of oomycetes. In both cases, plant identity and growth stage had little influence on the community structure of fungi and oomycetes, whereas soil/root compartment had a strong effect by filtering, resulting in a reduction of the number of microorganisms in roots as compared to the surrounding soil. The enrichment in roots with respect to soil of specific fungi and oomycetes, such as members of the Helotiales or the Peronosporales, suggests a specialization towards the asymptomatic colonization of plant tissues that could be relevant to host fitness and health.