Cellular localization of isoprenoid biosynthetic enzymes in Marchantia polymorpha. Uncovering a new role of oil bodies.
Cellular localization of isoprenoid biosynthetic enzymes in Marchantia polymorpha. Uncovering a new role of oil bodies.
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地钱中类异戊二烯生物合成酶的细胞定位。
DOI：
10.1104/pp.124.3.971
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发表时间：
2000
期刊：
影响因子：
7.4
通讯作者：
B. Camara
中科院分区：
文献类型：
--
作者：
C. Suire;F. Bouvier;R. Backhaus;D. Begu;M. Bonneu;B. Camara
像种子植物一样，地钱合成并积累了无数的类异戊二烯化合物。使用针对几个类异戊二烯生物合成酶的抗体，我们研究了它们的细胞内区室化原位免疫定位地钱。检测的酶是脱氧木酮糖磷酸合酶、香叶基二磷酸合酶、法呢基二磷酸合酶、香叶基香叶基二磷酸合酶、单萜合酶、香叶基香叶基二磷酸还原酶、八氢番茄红素合酶和八氢番茄红素去饱和酶。我们的研究结果表明，苔类油体，这是一个单一的单位膜结合的细胞器，具有类似的质体和胞质中发现的异戊二烯生物合成酶。我们假设，油体在细胞代谢中发挥着动态作用，除了他们的作用，作为网站的精油积累和隔离。这种酶在不同细胞区室中的出现可能是由于基因产物多靶向各种细胞器。
Like seed plants, liverworts synthesize and accumulate a myriad of isoprenoid compounds. Using antibodies raised against several isoprenoid biosynthetic enzymes, we investigated their intracellular compartmentation by in situ immunolocalization from Marchantia polymorpha. The enzymes examined were deoxy-xylulose phosphate synthase, geranyl diphosphate synthase, farnesyl diphosphate synthase, geranylgeranyl diphosphate synthase, monoterpene synthase, geranylgeranyl diphosphate reductase, phytoene synthase, and phytoene desaturase. Our results show that liverwort oil bodies, which are organelles bound by a single unit membrane, possess isoprenoid biosynthetic enzymes similar to those found in plastids and the cytosol. We postulate that oil bodies play a dynamic role in cell metabolism in addition to their role as sites of essential oil accumulation and sequestration. The occurrence of such enzymes in different cellular compartments might be due to multiple targeting of gene products to various organelles.
影响因子：
2.9
作者：
Williams, DC;McGarvey, DJ;Croteau, R
通讯作者：
Croteau, R
紫苏 4S-(-)-柠檬烯合酶的 cDNA 克隆、表征和功能表达。
DOI：
10.1006/abbi.1996.0343
发表时间：
1996
期刊：
Archives of biochemistry and biophysics.
影响因子：
--
作者：
Yuba,A;Yazaki,K;Tabata,M;Honda,G;Croteau,R
通讯作者：
Croteau,R