Angiotensin II increases pituitary cell prolactin release and arachidonate liberation.
Angiotensin II increases pituitary cell prolactin release and arachidonate liberation.
复制标题
血管紧张素 II 增加垂体细胞催乳素的释放和花生四烯酸的释放。
DOI：
10.1016/0303-7207(88)90040-8
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发表时间：
1988
影响因子：
4.1
通讯作者：
MacLeod,RM
中科院分区：
文献类型：
--
作者：
Judd,AM;Ross,PC;Spangelo,BL;MacLeod,RM
用[~H]花生四烯酸标记细胞磷脂，观察血管紧张素II(AII)对垂体前叶细胞释放催乳素和花生四烯酸的影响。AII在相似的浓度范围内增加催乳素释放和[~3H]花生四烯酸释放，这两个事件的动力学被证明是相同的。多巴胺抑制催乳素的释放和[~3H]花生四烯酸的释放。二酰甘油脂肪酶抑制剂RHC80267减少了All刺激的催乳素释放和花生四烯酸释放。进一步的证据表明，All诱导的花生四烯酸释放是由二酰基甘油脂肪酶介导的，这一发现表明，All增加了预先标记有脂肪酸的细胞的[14C]硬脂酸释放。虽然花生四烯酸本身可能对催乳素的分泌有一定的作用，但花生四烯酸代谢产物可能更直接地参与其中，因为花生四烯酸代谢产物形成的抑制剂BW755c和AA861增加了所有刺激的花生四烯酸的释放，但减少了催乳素的释放。
The effects of angiotensin II (AII) on prolactin release and arachidonate liberation were studied in anterior pituitary cells preincubated with [3H]arachidonate to label the cellular phospholipids. AII increased prolactin release and [3H]arachidonate liberation over similar concentration ranges with the dynamics of these two events proving identical. Dopamine attenuated both prolactin release and [3H]arachidonate liberation. The diacylglycerol lipase inhibitor RHC80267 decreased All-stimulated prolactin release and arachidonate liberation. Further evidence that All-induced release of arachidonate is mediated by a diacylglycerol lipase is suggested by the finding that All increased [14C]stearate liberation from cells prelabeled with the fatty acid. Although arachidonate itself may have some role in prolactin secretion, it is likely that arachidonate metabolites are more directly involved because BW755c and AA861, inhibitors of arachidonate metabolite formation, increased All-stimulated arachidonate liberation, but decreased prolactin release.