AUTORADIOGRAPHIC STUDY OF HISTOGENESIS IN MOUSE OLFACTORY BULB .I. TIME OF ORIGIN OF NEURONS AND NEUROGLIA
AUTORADIOGRAPHIC STUDY OF HISTOGENESIS IN MOUSE OLFACTORY BULB .I. TIME OF ORIGIN OF NEURONS AND NEUROGLIA
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DOI：
10.1002/cne.901340304
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发表时间：
1968-01-01
影响因子：
2.5
通讯作者：
HINDS, JW
中科院分区：
文献类型：
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作者：
HINDS, JW
用放射自显影确定小鼠嗅觉和副嗅觉结构的神经元和神经胶质细胞的起始时间(最终细胞分裂)。动物在不同发育阶段注射胸腺嘧啶核苷-H3，在成熟或接近成熟时被处死。在嗅觉结构中，二尖瓣细胞(最大的神经元)最先出现，主要出现在妊娠第11天至13天，簇状细胞主要出现在13天至18天，颗粒细胞(最小的神经元)主要出现在18天至出生后20天。大多数较小和较浅的外周丛生细胞出现得晚于更深和更大的中间和内部丛生细胞。这三种类型的颗粒细胞的起源时间都延长到出生后，内部颗粒细胞的出现时间比球周细胞或二尖瓣细胞层的颗粒细胞更长和更晚。神经胶质前体细胞主要在E17和P10之间进行最后的细胞分裂。在进化程度较低的副嗅觉结构中，二尖瓣细胞起源早于其同系物。二尖瓣细胞主要起源于E10~E12，颗粒细胞主要起源于E12~E18。研究结果支持这样一个概念，即中枢神经系统的一些生发层被编程为产生一系列细胞类型，即较大的细胞先于较小的细胞。
Time of origin (final cell division) of neurons and neuroglia of the mouse olfactory and accessory olfactory formations was determined by autoradiography. Animals were injected with thymidine‐H3at various developmental stages and killed at or near maturity. In the olfactory formation mitral cells (the largest neurons) arise first, mainly over the three day period from the eleventh day of gestation (E11) to E13, tufted cells chiefly from E13 to E18, and granule cells (the smallest neurons) mainly from E18 to postnatal day 20. Most of the smaller and more superficial peripheral tufted cells arise later than the deeper and larger middle and internal tufted cells. All three types of granule cells have a time of origin extending well into postnatal life, with internal granule cells arising over a longer and later period than periglomerular cells or granule cells of the mitral cell layer. Neuroglial precursors undergo final cell division chiefly between E17 and P10. In the phylogenetically less evolved accessory olfactory formation, mitral cells originate earlier than their homologues in the olfactory formation; mitral cells principally from E10 to E12 and granule cells chiefly from E12 to E18. The results support the concept that some germinal layers of the central nervous system are programmed to produce a succession of cell types, larger cells before smaller ones.