Exploiting Knowledge of R/Avr Genes to Rapidly Clone a New LZ-NBS-LRR Family of Late Blight Resistance Genes from Potato Linkage Group IV
Exploiting Knowledge of R/Avr Genes to Rapidly Clone a New LZ-NBS-LRR Family of Late Blight Resistance Genes from Potato Linkage Group IV
复制标题
DOI：
10.1094/mpmi-22-6-0630
复制
发表时间：
2009-06-01
影响因子：
3.5
通讯作者：
van der Vossen, Edwin A. G.
中科院分区：
文献类型：
--
作者：
Lokossou, Anoma A.;Park, Tae-ho;van der Vossen, Edwin A. G.
除了对致病疫霉（Phytophthora infestans，Rpi）基因Rpi-blb 1和Rpi-blb 2的抗性之外，Solanum bulbocastanum似乎具有位于LG IV上的主要晚疫病抗性基因座的Rpi-blb 3，该基因座在其他Solanum spp中也具有Rpi-abpt、R2、R2-like和Rpi-mcd 1。在这里，我们报告的4个Rpi基因的克隆和功能分析，使用基于图位的克隆方法，等位基因挖掘策略，网关技术，和瞬时互补分析在烟草本塞姆氏。Rpi-blb 3、Rpi-abpt、R2和R2-like包含亮氨酸拉链核苷酸结合位点富含亮氨酸重复序列（LZ-NBS-LRR）蛋白的所有特征序列，并且与来自拟南芥的RPP 13共享34.9%相似的氨基酸序列。所有四种Rpi蛋白的LRR结构域是高度同源的，而LZ和NBS结构域是更多态的，R2的那些是最不同的。四个功能性抗性蛋白和由另外的Rpi-blb 3基因同源物编码的蛋白之间的序列关联的清楚块表明LZ、NBS和LRR结构域的交换，强调了这些蛋白的模块化性质。所有四个Rpi基因识别最近鉴定的RXLR效应子PiAVR 2。
In addition to the resistance to Phytophthora infestans (Rpi) genes Rpi-blb1 and Rpi-blb2, Solanum bulbocastanum appears to harbor Rpi-blb3 located at a major late blight resistance locus on LG IV, which also harbors Rpi-abpt, R2, R2-like, and Rpi-mcd1 in other Solanum spp. Here, we report the cloning and functional analyses of four Rpi genes, using a map-based cloning approach, allele-mining strategy, Gateway technology, and transient complementation assays in Nicotiana benthamiana. Rpi-blb3, Rpi-abpt, R2, and R2-like contain all signature sequences characteristic of leucine zipper nucleotide binding site leucine-rich repeat (LZ-NBS-LRR) proteins, and share amino-acid sequences 34.9% similar to RPP13 from Arabidopsis thaliana. The LRR domains of all four Rpi proteins are highly homologous whereas LZ and NBS domains are more polymorphic, those of R2 being the most divergent. Clear blocks of sequence affiliation between the four functional resistance proteins and those encoded by additional Rpi-blb3 gene homologs suggest exchange of LZ, NBS, and LRR domains, underlining the modular nature of these proteins. All four Rpi genes recognize the recently identified RXLR effector PiAVR2.