Peptide-presenting two-dimensional protein matrix on supported lipid bilayers:: An efficient platform for cell adhesion
Peptide-presenting two-dimensional protein matrix on supported lipid bilayers:: An efficient platform for cell adhesion
复制标题
DOI：
10.1116/1.2821954
复制
发表时间：
2007-12-01
期刊：
影响因子：
2.1
通讯作者：
Brisson, Alain R.
中科院分区：
文献类型：
--
作者：
Berat, Remi;Remy-Zolghadry, Murielle;Brisson, Alain R.
了解和控制细胞在生物材料和合成材料上的粘附是基础研究和应用科学中的重要问题。用与脂质分子连接的细胞粘附肽功能化的支撑脂质双层（SLB）是流行的细胞粘附平台。在本文中，肽介绍的另一种方法，其中肽的立体选择性连接到蛋白质自组装在一个刚性的二维（2D）矩阵上的SLB。膜联蛋白-A5（Anx 5）因其在脂质表面形成稳定且刚性的2D基质的已知性质而被用作原型蛋白。合成了两种类型的Anx 5-肽复合物，其含有RGD或IKVAV序列。作者表明，这两个Anx 5-肽复合物呈现出相同的性质的结合和2D组织的脂质表面上的Anx 5，石英晶体微天平与耗散监测，原子力显微镜和透射电子显微镜技术进行调查时。发现Anx 5- RGD和Anx 5- IKVAV 2D基质分别促进人隐静脉内皮细胞和小鼠胚胎干细胞的特异性粘附。研究了暴露的肽的表面密度对细胞粘附的影响，表明当肽之间的平均距离小于约60 nm时，细胞附着于Anx 5-肽基质。这种细胞粘附平台提供了对细胞配体的方向和密度的控制，为未来的应用开辟了有趣的可能性。(c)2007年美国真空学会。
Understanding and controlling cell adhesion to biomaterials and synthetic materials are important issues in basic research and applied sciences. Supported lipid bilayers (SLBs) functionalized with cell adhesion peptides linked to lipid molecules are popular platforms of cell adhesion. In this paper, an alternative approach of peptide presentation is presented in which peptides are stereo-selectively linked to proteins self-assembling in a rigid two-dimensional (2D) matrix on SLBs. Annexin-A5 (Anx5) was used as prototype protein for its known properties of forming stable and rigid 2D matrices on lipid surfaces. Two types of Anx5- peptide complexes, containing either a RGD or an IKVAV sequence, were synthesized. The authors show that both Anx5- peptide complexes present the same properties of binding and 2D organization on lipid surfaces as Anx5, when investigated by quartz crystal microbalance with dissipation monitoring, atomic force microscopy, and transmission electron microscopy techniques. Anx5- RGD and Anx5- IKVAV 2D matrices were found to promote specific adhesion of human saphenous vein endothelial cells and mouse embryonic stem cells, respectively. The influence of the surface density of exposed peptides on cell adhesion was investigated, showing that cells attach to Anx5- peptide matrices when the average distance between peptides is smaller than about 60 nm. This cell adhesion platform provides control of the orientation and density of cell ligands, opening interesting possibilities for future applications.(c) 2007 American Vacuum Society.