Inositol cyclic triphosphate [inositol 1,2-(cyclic)-4,5-triphosphate] is formed upon thrombin stimulation of human platelets.
Inositol cyclic triphosphate [inositol 1,2-(cyclic)-4,5-triphosphate] is formed upon thrombin stimulation of human platelets.
复制标题
肌醇环三磷酸[肌醇1,2-(环)-4,5-三磷酸]是在凝血酶刺激人血小板时形成的。
DOI：
10.1073/pnas.83.17.6397
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发表时间：
1986
影响因子：
11.1
通讯作者：
Majerus,PW
中科院分区：
文献类型：
--
作者：
Ishii,H;Connolly,TM;Bross,TE;Majerus,PW
磷脂酶 C 在体外裂解多磷酸肌醇，导致形成环状和非环状肌醇磷酸。我们现已从凝血酶处理的血小板中分离出磷脂酰肌醇 4,5-二磷酸裂解的环状产物，即肌醇 1,2（环状）-4,5-三磷酸 [cIns(1:2,4,5)P3]。我们在凝血酶后 10 秒发现每 10(9) 个血小板有 0.2-0.4 nmol 的 cIns-(1:2,4,5)P3；在未刺激的血小板或添加凝血酶10分钟后的血小板中未发现任何变化。我们得出结论，cIns(1:2,4,5)P3 是凝血酶刺激的血小板中多磷酸肌醇代谢的主要产物。
Cleavage of polyphosphoinositides in vitro by phospholipase C results in formation of both cyclic and noncyclic inositol phosphates. We have now isolated the cyclic product of phosphatidylinositol 4,5-bisphosphate cleavage, inositol 1,2(cyclic)-4,5-triphosphate [cIns(1:2,4,5)P3], from thrombin-treated platelets. We found 0.2-0.4 nmol of cIns-(1:2,4,5)P3 per 10(9) platelets at 10 sec after thrombin; none was found in unstimulated platelets or in platelets 10 min after thrombin addition. We conclude that cIns(1:2,4,5)P3 is a major product of polyphosphoinositide metabolism in thrombin-stimulated platelets.