RNA PRIMER USED IN SYNTHESIS OF ANTICOMPLEMENTARY DNA BY REVERSE-TRANSCRIPTASE OF AVIAN-MYELOBLASTOSIS VIRUS
RNA PRIMER USED IN SYNTHESIS OF ANTICOMPLEMENTARY DNA BY REVERSE-TRANSCRIPTASE OF AVIAN-MYELOBLASTOSIS VIRUS
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DOI：
10.1073/pnas.77.3.1316
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
SPIEGELMAN, S
中科院分区：
文献类型：
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作者：
MYERS, JC;DOBKIN, C;SPIEGELMAN, S
当使用同源 RNA（禽类成髓细胞增多症病毒 RNA）或异源 RNA（脊髓灰质炎病毒 RNA）作为模板时，由禽类成髓细胞病病毒逆转录酶（RNA 指导的 DNA 核苷酸转移酶，EC 2.7.7.7）在体外合成的抗互补 DNA 由通常在 3'' 末端具有腺苷的原始 RNA 模板片段引发。当使用噬菌体 T4 RNA 连接酶 (EC 6.5.1.3) 标记 RNA .cntdot 中包含的 RNA 模板片段的 3'' 末端时。 c[互补]DNA杂合中间体，腺苷是携带标记的主要核苷。逆转录酶的 RNase H（混合核酸酶）活性产生原始 RNA 模板的片段，其中腺苷作为主要 3'' 末端，这些片段用作合成抗互补 DNA 的引物。
When either the homologous RNA (avian myeloblastosis virus RNA) or a heterologous RNA (poliovirus RNA) was used as a template, the anticomplementary DNA synthesized in vitro by avian myeloblastosis virus reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) was primed by fragments of the original RNA template that usually had adenosine at their 3'' ends. When phage T4 RNA ligase (EC 6.5.1.3) was used to label the 3'' end of the RNA template fragments contained in the RNA .cntdot. c[complementary]DNA hybrid intermediate, adenosine was the principal nucleoside carrying the label. The RNase H (hybrid nuclease) activity of the reverse transcriptase creates fragments of the original RNA template with adenosine as the principal 3'' terminus and that these fragments serve as primers for the synthesis of anticomplementary DNA.