Evaluation of the effect of a heat-killed lactic acid bacterium, Enterococcus faecalis 2001, on oral candidiasis
Evaluation of the effect of a heat-killed lactic acid bacterium, Enterococcus faecalis 2001, on oral candidiasis
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DOI：
10.3920/bm2018.0115
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发表时间：
2019-07
影响因子：
5.4
通讯作者：
T. Yamazaki;Ryoko Ushikoshi-Nakayama;K. Shirone;M. Suzuki;S. Abe;N. Matsumoto;H. Inoue;I. Saito
中科院分区：
文献类型：
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作者：
T. Yamazaki;Ryoko Ushikoshi-Nakayama;K. Shirone;M. Suzuki;S. Abe;N. Matsumoto;H. Inoue;I. Saito
两项研究评估了热灭活革兰氏阳性乳酸菌粪肠球菌 2001 (EF-2001) 制剂对口腔念珠菌病的作用。进行了一项体外研究，以评估从口腔念珠菌病患者分离的念珠菌菌株对菌丝生长的抑制作用，并在口腔念珠菌病患者中进行了临床研究。在体外研究中，EF-2001 抑制 IT-1（一种从口腔念珠菌病患者分离出的念珠菌菌株）的菌丝生长，浓度≥2.34×109 个细胞/ml。对 13 名口腔念珠菌病患者进行了一项开放临床研究。受试者每天睡前服用一次含有7.5×1011热灭活EF-2001的粉末，连续7天。在可供分析的 12 名患者中，有 11 名 (92%) 的口腔念珠菌载量 (cfu/拭子) 显示显着下降 (P=0.01079，d=-0.437)。白色念珠菌减少了 55%，光滑念珠菌减少了 93.8%。以下症状有显着改善：耳鸣（P=0.048，d=-0.462）、冷感（P=0.048，d=-0.463）和抑郁症（P=0.019，d=-0.34）。此外，26 种口腔症状中有 4 种有改善的趋势。这些结果表明，EF-2001 通过抑制菌丝生长，显着降低口腔念珠菌病患者的口腔念珠菌负荷，EF-2001 是口腔念珠菌病的有效治疗方法。
The effect of a preparation of heat-killed Gram-positive lactic acid bacteria Enterococcus faecalis 2001 (EF-2001) on oral candidiasis was evaluated by two studies. An in vitro study was performed to assess the inhibitory effect on mycelial growth of Candida strains isolated from a patient with oral candidiasis, and a clinical study was done in patients with oral candidiasis. In the in vitro study, EF-2001 inhibited mycelial growth of IT-1, a Candida strain isolated from a patient with oral candidiasis, at concentrations ≥2.34×109 cells/ml. An open clinical study was performed in 13 patients with oral candidiasis. The subjects took a powder containing 7.5×1011 heat-killed EF-2001 once a day before bedtime for seven consecutive days. In 11 of the 12 patients available for analysis (92%), the oral Candida load (cfu/swab) showed a significant decrease (P=0.01079, d=-0.437). There was a 55% decrease of Candida albicans and a 93.8% decrease of Candida glabrata. The following symptoms showed significant improvement: tinnitus (P=0.048, d=-0.462), cold feeling (P=0.048, d=-0.463), and depression (P=0.019, d=-0.34). In addition, 4 out of 26 oral symptoms tended to improve. These results suggest that EF-2001 significantly decreased the oral Candida load in patients with oral candidiasis by inhibiting mycelial growth and that EF-2001 is an effective treatment for oral candidiasis.