Identification of a Receptor-Binding Domain in the S Protein of the Novel Human Coronavirus Middle East Respiratory Syndrome Coronavirus as an Essential Target for Vaccine Development
Identification of a Receptor-Binding Domain in the S Protein of the Novel Human Coronavirus Middle East Respiratory Syndrome Coronavirus as an Essential Target for Vaccine Development
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DOI：
10.1128/jvi.01048-13
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发表时间：
2013-09-01
影响因子：
5.4
通讯作者：
Jiang, Shibo
中科院分区：
文献类型：
--
作者：
Du, Lanying;Zhao, Guangyu;Jiang, Shibo
一种新型人类中东呼吸综合征冠状病毒（MERS - CoV）引发了类似严重急性呼吸综合征（SARS）的疾病暴发，死亡率较高，引发了人们对其大流行潜力的担忧。二肽基肽酶 - 4（DPP4）近期被确定为其受体。在此我们表明，MERS - CoV的S蛋白中第377至662位氨基酸残基特异性地结合表达DPP4的细胞以及可溶性DPP4蛋白，并诱导显著的中和抗体反应，这表明该区域包含受体结合域（RBD），其有潜力被开发为一种MERS - CoV疫苗。
A novel human Middle East respiratory syndrome coronavirus (MERS-CoV) caused outbreaks of severe acute respiratory syndrome (SARS)-like illness with a high mortality rate, raising concerns of its pandemic potential. Dipeptidyl peptidase-4 (DPP4) was recently identified as its receptor. Here we showed that residues 377 to 662 in the S protein of MERS-CoV specifically bound to DPP4-expressing cells and soluble DPP4 protein and induced significant neutralizing antibody responses, suggesting that this region contains the receptor-binding domain (RBD), which has a potential to be developed as a MERS-CoV vaccine.