The ABC transporter ABCH-9C is needed for cuticle barrier construction in Locusta migratoria
The ABC transporter ABCH-9C is needed for cuticle barrier construction in Locusta migratoria
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飞蝗角质层屏障构建需要 ABC 转运蛋白 ABCH-9C
DOI：
10.1016/j.ibmb.2017.06.005
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发表时间：
2017
影响因子：
3.8
通讯作者：
Zhang Jianzhen
中科院分区：
文献类型：
--
作者：
Yu Zhitao;Wang Yiwen;Zhao Xiaoming;Liu Xiaojian;Ma Enbo;Moussian Bernard;Zhang Jianzhen
ATP结合盒（ABC）转运蛋白是一个蛋白质超家族，介导多种底物的跨膜转运，包括营养物质、脂质和外源性物质，发挥多种发育和生理功能。本文报道了蝗虫（Locusta migratoria）ABC转运蛋白LmABCH-9 C的分子特性和生物学功能，LmABCH-9 C在蝗虫若虫发育过程中，在包括体壁在内的所有组织中持续表达。蜕皮后表达量最高。通过RNA干扰（RNAi）抑制LmABCH-9 C转录水平，在蜕皮期间或蜕皮后不久引起死亡。它们在蜕皮后几分钟内体重就减轻了。此外，高湿度挽救了蜕皮的dsLmABCH-9 C注射的果蝇的致死率。在组织学实验中，我们发现，在LmABCH-9 C表达受抑制的大鼠中，内表皮脂质的量减少。这些数据共同表明LmABCH-9 C是脂质依赖性脱水抗性所必需的，与ABCH-9 C的功能相似。因此，这种ABC转运蛋白的功能似乎在从半代谢（L. migratoria）到全代谢（T. castaneum）物种的昆虫物种中是保守的。此外，我们发现，角质层向内的不渗透性是受损的，在lmABCH-9 C功能降低的皮肤。总之，与角质层脂质是防止昆虫干燥和外源性物质渗透所必需的模型一致，我们假设LmABCH-9 C参与角质层表面脂质屏障的构建，特别是在蜕皮后，以保护动物免受不受控制的水分损失和进入。这种ABC转运蛋白对RNAi介导的敲低的敏感性使其成为基于RNAi的昆虫害虫控制的极好靶标。
ATP-binding cassette (ABC) transporters constitute a large superfamily of proteins that mediate transport of a diverse number of substrates including nutrients, lipids and xenobiotics across membranes serving a variety of developmental and physiological functions. Here, we report on the molecular properties and biological roles of the ABC transporterLmABCH-9Cin the migratory locustLocusta migratoria.LmABCH-9Cwas expressed continuously during nymphal development in all tissues including the integument. Expression was highest just after molting. Suppression ofLmABCH-9Ctranscript levels by RNA interference (RNAi) in nymphs provoked death during or soon after molting to the next stage. These nymphs lost weight within minutes after molting. Moreover, high humidity rescued the lethality of molted dsLmABCH-9C-injected nymphs. In histological experiments, we find that the amounts of inner-cuticular lipids are reduced in nymphs with suppressedLmABCH-9Cexpression. These data together indicate thatLmABCH-9Cis needed for lipid-dependent desiccation resistance, paralleling the function ofABCH-9CinTriboliumcastaneum. Hence, the function of this ABC transporter seems to be conserved across insect species ranging from hemimetabolous (L. migratoria) to holometabolous (T. castaneum) species. In addition, we find that cuticle inward impermeability is compromised in nymphs with reducedLmABCH-9Cfunction. In summary, consistent with the model that cuticular lipids are necessary to prevent desiccation and penetration of xenobiotics in insects, we hypothesize thatLmABCH-9Cis involved in the construction of a lipid-based barrier at the surface of the cuticle especially after molting to protect the animal against uncontrolled water loss and entry. Susceptibility of this ABC transporter to RNAi-mediated knockdown designates it as an excellent target for RNAi-based insect pest control.