Senile changes in the ganglion cells of the human retina.
Senile changes in the ganglion cells of the human retina.
复制标题
人类视网膜神经节细胞的老年变化。
DOI：
--
复制
发表时间：
1965
影响因子：
4.1
通讯作者：
F. Vrabec
中科院分区：
文献类型：
--
作者：
F. Vrabec
材料与方法对16例成人视网膜标本进行了研究。用Gros-Schultze方法浸渍视网膜固定片，该方法稍有改进，将在20%硝酸银浴中的浸渍时间从1 ~ 2小时缩短到10 ~ 15分钟。研究了人类视网膜从第四个到第九个十年，十年是在70和85岁之间。浸渍后，将整个视网膜（在周边的四个斜子午线上有交叉切口以便于压平）固定在加拿大香脂中，内表面最高。其他脊椎动物的几个视网膜进行了比较研究。由于视网膜是完整安装的，因此避免了因破裂而产生伪影的危险。通过将显微镜聚焦于内丛状层的特定层，可以研究大的、弥漫的神经节细胞的树突，这些神经节细胞水平分布在广阔的区域，但是垂直定向的树突只能通过一些困难来观察。具有较长工作距离的高倍率干透镜最适用于这项工作。在树脂固化过程中，使用小重量对支架进行轻微压缩，可以研究轴突和树突的细节。在冷冻切片机上将同一只眼睛的前段切成厚切片（60至100 μ），并通过原始的Gros-Schultze方法浸渍。
Material and Methods Whole mounts of sixteen human adult retinae were studied. Retinal mounts were impregnated by the method of Gros-Schultze, which was slightly modified by shortening the time of impregnation in the 20 per cent. silver nitrate bath to 10 to 15 minutes instead of 1 to 2 hours. Human retinae from the fourth to the ninth decade were studied, ten being between 70 and 85 years. After impregnation, the whole retinae (with cross incisions in the four oblique meridians at the periphery to facilitate flattening) were mounted in Canada balsam, with the inner surface uppermost. Several retinae of other vertebrates were studied for comparison. As the retinae were mounted whole, the danger of artefacts due to breakage was avoided. It was possible, by focusing the microscope upon a particular lamina of the inner plexiform layer, to study the dendrites of large, diffuse ganglion cells spreading horizontally over wide areas, but vertically orientated dendrites could be followed only with some difficulty. High-power dry lenses with longer working distance were most useful for this work. A light compression of the mount, using a small weight during the setting of the resin, permitted the study of fine details of axons and dendrites. Anterior segments of the same eyes were cut in thick sections (60 to 100 ,u) on the freezing microtome and impregnated by the original Gros-Schultze method.