Fabrication of ‘Clickable’ Polyfluorene Nanowires with High Aspect Ratio as Biological Sensing Platforms
Fabrication of ‘Clickable’ Polyfluorene Nanowires with High Aspect Ratio as Biological Sensing Platforms
复制标题
制造具有高纵横比的“可点击”聚芴纳米线作为生物传感平台
DOI：
10.1021/acssensors.6b00070
复制
发表时间：
2016
期刊：
影响因子：
8.9
通讯作者：
S. Seki
中科院分区：
文献类型：
--
作者：
T. Wasin;K. Enomoto;T. Sakurai;V. Padalkar;H. Cheng;M. Tang;A. Horio;D.;Sakamaki;M. Omichi;A. Saeki;K. Kikuchi;Y. Hori;A. Chiba;Y. Saito;T. Kamiya;M. Sugimoto;S. Seki
利用单粒子纳米纤维技术（SPNT）成功制备了具有良好结构和均匀结构的共轭聚芴聚合物“可点击”纳米线。聚[（9，9-二己-5-炔-1-基芴基-2，7-二基）-co-（9，9 ′-二正辛基芴基-2，7-二基）]（F6 E8）和聚[（9，9-二己-5-炔-1-基芴基-2，7-二基）-co-（2，2 ′-联噻吩）]（F6 E2 T）在辐照后发生有效的交联反应，导致形成具有高达200的高且期望的纵横比的一维纳米结构。通过与荧光5-TAMRA-PEG 3-叠氮化物的点击反应，纳米线表面上的炔基被有效地官能化，这通过共聚焦显微镜证实。用纳米线官能化的基底提供直接用塔姆拉固定的“可点击”表面积的急剧膨胀，并且塔姆拉和纳米线主链之间的荧光共振能量转移（FRET）过程被证明是生物传感平台。
“Clickable” nanowires with well-defined and uniform structures made of conjugated polyfluorene polymers were successfully fabricated by single particle nanofabrication technique (SPNT). Poly[(9,9-dihex-5-yn-1-ylfluorenyl-2,7-diyl)-co-(9,9′-di-n-octylfluorenyl-2,7-diyl)] (F6E8) and poly[(9,9-dihex-5-yn-1-ylfluorenyl-2,7-diyl)-co-(2,2′-bithiophene)] (F6E2T) underwent an efficient cross-linking reaction upon irradiation, resulting in formation of one-dimensional nanostructures with high and desired aspect ratio reaching up to 200. Alkyne groups on the surface of nanowires were functionalized effectively by click reaction with fluorescent 5-TAMRA-PEG3-azide, which was confirmed by confocal microscopy. Substrates functionalized with the nanowires provide dramatic expansion of “clickable” surface area immobilized directly with TAMRA, and the fluorescence resonance energy transfer (FRET) processes between TAMRA and nanowire backbones are demonstrated as biological sensing platforms.