ANALYSIS OF INTERSTRAIN VARIATION IN CYTOMEGALOVIRUS GLYCOPROTEIN-B SEQUENCES ENCODING NEUTRALIZATION-RELATED EPITOPES
ANALYSIS OF INTERSTRAIN VARIATION IN CYTOMEGALOVIRUS GLYCOPROTEIN-B SEQUENCES ENCODING NEUTRALIZATION-RELATED EPITOPES
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DOI：
10.1093/infdis/163.6.1229
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发表时间：
1991-06-01
影响因子：
6.4
通讯作者：
DENNISON, KM
中科院分区：
文献类型：
--
作者：
CHOU, SW;DENNISON, KM
应用聚合酶链反应（PCR）技术，扩增了12株不同临床分离株的人巨细胞病毒（CMV）包膜糖蛋白B（gB）基因的一部分核苷酸序列，该基因编码病毒中和性单克隆抗体识别的表位。该区域（密码子384-717）的序列分析显示，临床菌株和先前测序的实验室菌株Towne和AD 169属于四个变异组之一，每个变异组具有特征性核苷酸和肽序列。肽同源性对于组内的菌株> 99%，并且对于不同组中的菌株在91%至98%之间变化。密码子448和480之间的变异最频繁。CMV毒株的gB组可以通过从病毒基因组DNA扩增的小靶序列的限制性分析来确定，并且另外28个临床毒株以这种方式分组。临床菌株中存在有限数量的gB变异体有助于分析生物学功能和免疫反应的交叉反应性。
Nucleotide sequences of a part of the envelope glycoprotein B (gB) gene of human cytomegalovirus (CMV), encoding epitopes recognized by virus-neutralizing monoclonal antibodies, were determined for 12 distinct clinical strains of CMV after amplification of suitable templates using the polymerase chain reaction. Sequence analysis of this region (codons 384-717) revealed that the clinical strains and previously sequenced laboratory strains Towne and AD169 belong to one of four variant groups, each with a characteristic nucleotide and peptide sequence. Peptide homology was > 99% for strains within a group, and varied from 91% to 98% for strains in different groups. Variation was most frequent between codons 448 and 480. The gB group of a CMV strain could be determined by restriction analysis of a small target sequence amplified from viral genomic DNA, and an additional 28 clinical strains were grouped in this manner. The existence of a limited number of variants of gB among clinical strains facilitates analysis of biologic function and cross-reactivity of immune responses.