The Effects of Olive Leaf Extract on The Testis, Sperm Quality and Testicular Germ Cell Apoptosis in Male Rats Exposed to Busulfan
The Effects of Olive Leaf Extract on The Testis, Sperm Quality and Testicular Germ Cell Apoptosis in Male Rats Exposed to Busulfan
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DOI：
10.22074/ijfs.2019.5520
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发表时间：
2019-04-01
影响因子：
2.5
通讯作者：
Eftekhar-Vaghefi, Seyed Hassan
中科院分区：
文献类型：
--
作者：
Hakemi, Sepideh Ganjalikhan;Sharififar, Fariba;Eftekhar-Vaghefi, Seyed Hassan
背景：白消安（BU）对男性生殖系统有破坏作用。本研究的目的是评估橄榄叶提取物（OLE）作为抗氧化剂和酚类化合物的来源，对BU诱导的大鼠testes.Materials和方法的损害的影响：在本实验研究中，40只雄性Wistar大鼠随机分为5组。对照组（CTL）接受单次腹膜内（i. p.）注射二甲基亚砜（DMSO），然后口服蒸馏水5周。BU组腹腔注射BU（10 mg/kg）1次。联合治疗组先腹腔注射BU（10 mg/kg），然后口服OLE（BU+OLE 250）、500 mg/kg（BU +OLE 500）和750 mg/kg（BU +OLE 750），连续5周。接下来，收集血液和精子样本。取左侧睾丸，分别用H&E和TUNEL染色检测睾丸参数和细胞凋亡。所有数据用SPSS软件进行统计分析，P
Background: Busulfan (BU) has a destructive effect on the male reproductive system. The goal of this study was to assess the effects of olive leaf extract (OLE) as a source of antioxidants and phenolic compounds, on BU-induced damages in rat testes.Materials and Methods: In this experimental study, 40 male Wistar rats were randomly divided into 5 groups. The control group (CTL) received a single intraperitoneal (i.p.) injection of dimethyl sulfoxide (DMSO), followed by oral administration of distilled water for 5 weeks. In BU group, BU (10 mg/kg) was administrated i.p. once. In cotreatment groups, first, received BU (10 mg/kg, a single i.p. injection) then, OLE was administrated orally at different doses of 250 mg/kg (BU+OLE 250), 500 mg/kg (BU+OLE 500) and 750 mg/kg (BU+OLE 750), for 5 weeks. Next, blood and sperm samples were collected. The left testis was removed to investigate testicular parameters and apoptosis by using H&E and TUNEL staining, respectively. All data were analyzed by SPSS software and a P