Use of in vitro (Ames and Mutatox tests) and in vivo (Amphibian Micronucleus test) assays to assess the genotoxicity of leachates from a contaminated soil
Use of in vitro (Ames and Mutatox tests) and in vivo (Amphibian Micronucleus test) assays to assess the genotoxicity of leachates from a contaminated soil
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DOI：
10.1016/s0146-6380(99)00079-0
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发表时间：
1999-01-01
影响因子：
3
通讯作者：
Vasseur, P
中科院分区：
文献类型：
--
作者：
Békaert, C;Rast, C;Vasseur, P
采用两种体外细菌试验（艾姆斯试验和Mutatox试验）和一种两栖动物体内微核试验，对受多环芳烃（PAHs）和重金属污染的土壤样品水沥滤液的遗传毒性进行了评价。三项试验均获得阳性结果：表明该污染土壤可能对水生环境构成威胁。试验灵敏度受实验条件的影响。与0.45 μ m过滤的一个，这是标准协议（规范X31-210）的响应是较高的未过滤的渗滤液。必须重新考虑这一标准程序，以便说明吸附在具有生物活性的固体颗粒上的污染物。与琼脂平板法相比，在液体培养基中培养细菌可提高污染物的生物利用度，从而提高艾姆斯试验的灵敏度。生物测试似乎是对物理化学分析的有益补充，因为它们说明了化学品的生物利用率和生物累积性以及污染物之间的相互作用。(C)1999爱思唯尔科技有限公司。保留所有权利。
Two in vitro bacterial assays (Ames test and Mutatox test) and one in vivo Micronucleus test on amphibians were used to assess the genotoxicity of aqueous leachates from a soil sample contaminated with polycyclic aromatic hydrocarbons (PAHs) and heavy metals. Positive results were obtained with the three tests: suggesting that this contaminated soil may be a threat to the aquatic environment. The test sensitivity was shown to be influenced by the experimental conditions. The response was higher with the nonfiltered leachate than with 0.45 mu m filtered one, which was the standard protocol (Norm X31-210). This standard procedure must be reconsidered in order to account for the pollutants adsorbed on solid particles which were biologically active. Incubation of bacteria in a liquid medium enhanced the sensitivity of the Ames test, by increasing the bioavailability of pollutants in comparison with the agar plate method. Biological tests appeared to be a useful complement to physico-chemical analysis, since they account for bioavailability and bioaccumulation of chemicals, and interactions between pollutants. (C) 1999 Elsevier Science Ltd. All rights reserved.