Induction of histidine decarboxylase of rat basophilic leukemia (2H3) cells stimulated by higher oligomeric IgE or phorbol myristate acetate.
Induction of histidine decarboxylase of rat basophilic leukemia (2H3) cells stimulated by higher oligomeric IgE or phorbol myristate acetate.
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由较高寡聚 IgE 或佛波醇肉豆蔻酸酯乙酸酯刺激的大鼠嗜碱性白血病 (2H3) 细胞诱导组氨酸脱羧酶。
DOI：
10.1016/s0006-291x(88)80518-7
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发表时间：
1988
影响因子：
3.1
通讯作者：
Takehiko Watanabe
中科院分区：
文献类型：
--
作者：
Kazutaka Maeyama;Y. Taguchi;M. Sasaki;Hiroshi Wada;M. A. Beaven;Takehiko Watanabe
当大鼠嗜碱性白血病（2H3）细胞受到较高的低聚物（IgE的化学交联低聚物）刺激时，在钙的存在下，组胺形成酶组氨酸脱羧酶（HDC， l -组氨酸羧化酶，E.C.4.1.1.22）的活性增加1小时，2小时达到最大活性，8小时恢复到原始水平。用肉豆蔻酸酯（PMA）或油基乙酰甘油（OAG）处理的细胞也观察到类似的酶活性增加，这两种物质是已知的蛋白激酶c的激活剂。从培养基中去除钙可以消除高寡聚物引起的HDC活性的增加，但不影响PMA或OAG引起的HDC活性的增加，这表明HDC活性的增加可能是由蛋白激酶c介导的。因为它被环己亚胺阻止了。
When rat basophilic leukemia (2H3) cells were stimulated by higher oligomer, the chemically cross-linked oligomers of IgE, in the presence of calcium the activity of histidine decarboxylase (HDC, L-histidine carboxylyase, E.C.4.1.1.22), a histamine-forming enzyme, was increased by 1 hr, reaching maximum activity by 2 hr, and returning to the original level by 8 hr. A similar increase in enzyme activity was observed in cells treated with phorbol myristate acetate (PMA) or oleoyl-acetylglycerol (OAG), which are known activators of protein kinase C. Removal of calcium from medium abolished the increase in HDC activity in response to higher oligomer but not that induced by PMA or OAG, suggesting that the increase in HDC activity may be mediated by protein kinase C. The increase in the HDC activity probably required induction of enzyme synthesis, because it was prevented by cycloheximide.