Putative allergic reactivity of casein phosphopeptide in severe cow’s milk allergy patients
Putative allergic reactivity of casein phosphopeptide in severe cow’s milk allergy patients
复制标题
严重牛奶过敏患者酪蛋白磷酸肽的推定过敏反应
DOI：
10.1111/pai.13752
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发表时间：
2022
影响因子：
4.4
通讯作者：
Ito Komei
中科院分区：
文献类型：
--
作者：
Matsui Teruaki;Naito Michihiro;Kitamura Katsumasa;Makino Atsushi;Takasato Yoshihiro;Sugiura Shiro;Izumi Hidehiko;Ito Komei
关键词：嗜碱性粒细胞；酪蛋白；牛奶；食物过敏；牛奶过敏；牛奶过敏；嗜碱性粒细胞酪蛋白牛奶食物过敏牛奶过敏乳多肽1 5 5 03/29/22 20220301 NES 220301认可我们感谢所有从事OFCS和数据收集工作的医生、营养师和护士以及早川信行先生(爱知儿童保健医疗中心临床实验室技术员)和松尾久美子女士(BML，Inc.细胞生物科)。感谢他们出色的技术援助。首先，服用含有糊剂或口香糖的CPP-ACP后出现的症状可能不是CPP-ACP引起的，因为使用CPP-ACP的OFC不进行，OFC到发病的时间有时很长，并且用CPP代替CPP-ACP进行体外I试验。我们招募了6名CMA患者，他们的严重程度与CPP-ACP相似(反应10毫升CM)，他们的酪蛋白特异性IgE滴度水平超过两位数，并完全避免了体外试验中的CM(表S1)。第三，我们实验中使用的CPP试剂是胰酶消化的酪蛋白多肽的混合物，它可能包含残留的蛋白质IgE表位，以及酪蛋白中的CPP区域。[摘自文章]儿科过敏和免疫的版权属于Wiley-Blackwell的财产，其内容不得复制或通过电子邮件发送到多个网站或发布到列表服务器上，除非获得版权所有者的明确书面许可。但是，用户可以打印、下载或通过电子邮件发送文章供个人使用。这篇摘要可以删节。对复制品的准确性不作任何保证。用户应参考该材料的原始出版版本，以获取完整摘要。版权适用于所有摘要。
Keywords: basophils; casein; cow milk; food allergy; milk hypersensitivity; phosphopeptide EN basophils casein cow milk food allergy milk hypersensitivity phosphopeptide 1 5 5 03/29/22 20220301 NES 220301 ACKNOWLEDGMENTS We thank all the physicians, nutritionists, and nurses who performed the OFCs and data collection and Mr. Noboru Hayakawa (Clinical Laboratory technician in Aichi Children's Health and Medical Center) and Ms. Kumiko Matsuo (Cell Biology Section, BML, Inc.) for their excellent technical assistance. First, symptoms that developed after ingesting CPP-ACP containing paste or gum were possibly not induced by CPP-ACP because OFC using CPP-ACP was not performed, the time between OFC and episode was sometimes long, and I in vitro i tests were performed with CPP instead of CPP-ACP. We recruited six patients with CMA whose severity was similar to that of CPP-ACP (reacted < 10 ml of CM) and who presented with casein-specific IgE titer levels above two digits and completely avoided CM for the in vitro tests (Table S1). Third, the CPP reagent used in our experiments was a mixture of trypsin-digested casein peptides, which might contain residual protein IgE epitopes, along with the CPP region in casein.[Extracted from the article]Copyright of Pediatric Allergy & Immunology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. Copyright applies to all Abstracts.