Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata.
Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata.
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DOI：
10.1038/s41598-017-01961-4
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发表时间：
2017-05-11
影响因子：
4.6
通讯作者：
Donly C
中科院分区：
文献类型：
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作者：
Kaplanoglu E;Chapman P;Scott IM;Donly C
目前的害虫控制依赖于化学杀虫剂，然而，害虫的杀虫剂抗性发展是害虫管理中日益关注的问题。杀虫剂抗性的主要机制通常涉及解毒酶和分解和排泄杀虫剂分子的异生素转运蛋白的活性升高。在这项研究中，我们调查了吡虫啉抗性的分子机制，在科罗拉多马铃薯甲虫，Leptinotarsa decemlineata（说）（鞘翅目：夜蛾科），一种昆虫害虫，其能力发展迅速的杀虫剂抗性。我们比较了吡虫啉抗性和敏感甲虫菌株的转录组图谱，并确定了102个差异表达的转录本编码解毒酶和异源生物质转运蛋白。其中，74个在抗性菌株中上调，28个在抗性菌株中下调。然后，我们使用RNA干扰来敲低抗性甲虫中七个上调基因的转录水平。摄入双链RNA成功地敲低了三种细胞色素P450（CYP 6 BQ 15、CYP 4 Q3和CYP 4 Q7）、一种ATP结合盒（ABC）转运蛋白（ABC-G）、一种酯酶（EST 1）和两种UDP-糖基转移酶（UGT 1和UGT 2）的基因表达。此外，我们证明了CYP 4 Q3和UGT 2的沉默显着增加抗性甲虫对吡虫啉的敏感性，表明这两个基因的过表达有助于该抗性品系对吡虫啉的抗性。
Current control of insect pests relies on chemical insecticides, however, insecticide resistance development by pests is a growing concern in pest management. The main mechanisms for insecticide resistance typically involve elevated activity of detoxifying enzymes and xenobiotic transporters that break-down and excrete insecticide molecules. In this study, we investigated the molecular mechanisms of imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), an insect pest notorious for its capacity to develop insecticide resistance rapidly. We compared the transcriptome profiles of imidacloprid-resistant and sensitive beetle strains and identified 102 differentially expressed transcripts encoding detoxifying enzymes and xenobiotic transporters. Of these, 74 were up-regulated and 28 were down-regulated in the resistant strain. We then used RNA interference to knock down the transcript levels of seven up-regulated genes in the resistant beetles. Ingestion of double-stranded RNA successfully knocked down the expression of the genes for three cytochrome P450s (CYP6BQ15, CYP4Q3 and CYP4Q7), one ATP binding cassette (ABC) transporter (ABC-G), one esterase (EST1), and two UDP-glycosyltransferases (UGT1 and UGT2). Further, we demonstrated that silencing of CYP4Q3 and UGT2 significantly increased susceptibility of resistant beetles to imidacloprid, indicating that overexpression of these two genes contributes to imidacloprid resistance in this resistant strain.