TRANSIENT BLOOD-BRAIN BARRIER PERMEABILITY FOLLOWING PROFOUND TEMPORARY GLOBAL-ISCHEMIA - AN EXPERIMENTAL-STUDY USING C-14 AIB
TRANSIENT BLOOD-BRAIN BARRIER PERMEABILITY FOLLOWING PROFOUND TEMPORARY GLOBAL-ISCHEMIA - AN EXPERIMENTAL-STUDY USING C-14 AIB
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DOI：
10.1038/jcbfm.1989.10
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发表时间：
1989-02-01
影响因子：
6.3
通讯作者：
ROSSRUSSELL, R
中科院分区：
文献类型：
--
作者：
DOBBIN, J;CROCKARD, HA;ROSSRUSSELL, R
研究了雄性Sprague-Dawley大鼠重度不完全前脑缺血后再灌注对血脑屏障（BBB）对小蛋白示踪剂渗透性的影响。14c -氨基异丁酸（AIB）的平均皮质血脑转移常数（Ki）在再灌注3和6 h时显著高于对照组，为其平均值的2.5倍（p < 0.05）。L g-1 min-1和1.0 .mu。但在再灌注24小时后恢复到控制值。Ki值分布分析显示，在深度缺血15分钟和30分钟后，再循环长达6小时后，AIB通过血脑屏障（BBB）的转移显著增加，尽管Evans Blue （EB）染料评估没有发现蛋白质外渗的证据。再灌注24h后血脑屏障到AIB恢复，Ki值恢复到对照值。结论：短暂性全脑缺血后，血脑屏障可迅速恢复。
The influence of reperfusion after profound incomplete forebrain ischaemia on blood-brain barrier (BBB) permeability to a small protein tracer was studied in male Sprague-Dawley rats. The mean cortical blood to brain transfer constant (Ki) for 14C-amino isobutyric acid (AIB) was significantly greater at 3 and 6 h of reperfusion, 2.5 times the mean values of controls (p < 0.05) (2.5 .mu.l g-1 min-1 and 1.0 .mu.l g-1 min-1 respectively), but had returned to control values after reperfusion for 24 h. Analysis of distribution of Ki values showed that following 15 min and 30 min of profound ischaemia, there was a significant increase in transfer of AIB across the blood-brain barrier (BBB) after recirculation for up to 6 h, though there was no evidence of protein extravasation as assessed by Evans Blue (EB) dye. After 24 h of reperfusion, the BBB to AIB was restored, and Ki values had returned to control values. It is concluded that following transient global ischaemia, the BBB may recover rapidly.