FtsY, the Prokaryotic Signal Recognition Particle Receptor Homologue, Is Essential for Biogenesis of Membrane Proteins*
FtsY, the Prokaryotic Signal Recognition Particle Receptor Homologue, Is Essential for Biogenesis of Membrane Proteins*
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DOI：
10.1074/jbc.272.4.2053
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发表时间：
1997-01
期刊：
影响因子：
--
通讯作者：
A. Seluanov;E. Bibi
中科院分区：
文献类型：
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作者：
A. Seluanov;E. Bibi
在哺乳动物细胞中，许多分泌蛋白通过信号识别颗粒（SRP）及其受体协同作用靶向内质网。在大肠杆菌中，分泌性蛋白质靶向内膜可以在分泌后完成。出乎意料的是，尽管有这种差异，E。大肠杆菌含有编码Ffh和FtsY的必需基因，其与真核生物SRP机制的蛋白质具有显著的相似性。在这项研究中，我们调查的可能性，原核生物的SRP样机制参与的膜蛋白的生物合成在E。杆菌本文提供的数据表明，SRP受体同源物，FtsY，确实是必不可少的表达膜蛋白在大肠杆菌。这表明，在这组蛋白质的情况下，FtsY和哺乳动物SRP受体具有相似的功能。
In mammalian cells, many secretory proteins are targeted to the endoplasmic reticulum co-translationally, by the signal recognition particle (SRP) and its receptor. In Escherichia coli, the targeting of secretory proteins to the inner membrane can be accomplished post-translationally. Unexpectedly, despite this variance, E. coli contains essential genes encoding Ffh and FtsY with a significant similarity to proteins of the eukaryotic SRP machinery. In this study, we investigated the possibility that the prokaryotic SRP-like machinery is involved in biogenesis of membrane proteins in E. coli. The data presented here demonstrate that the SRP-receptor homologue, FtsY, is indeed essential for expression of integral membrane proteins in E. coli, indicating that, in the case of this group of proteins, FtsY and the mammalian SRP receptor have similar functions.