DegU and YczE positively regulate the synthesis of bacillomycin D by Bacillus amyloliquefaciens strain FZB42
DegU and YczE positively regulate the synthesis of bacillomycin D by Bacillus amyloliquefaciens strain FZB42
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DOI：
10.1128/aem.00565-07
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发表时间：
2007-11-01
影响因子：
4.4
通讯作者：
Borriss, Rainer
中科院分区：
文献类型：
--
作者：
Koumoutsi, Alexandra;Chen, Xiao-Hua;Borriss, Rainer
环境菌株解淀粉芽孢杆菌FZB42不同于同属的驯化模式生物枯草芽孢杆菌168，其促进植物生长和抑制根际存在的植物病原生物的能力不同。这种行为主要是通过产生几种非核糖体环状脂肽和聚酮来发挥作用的，它们对植物致病菌、真菌和线虫具有广泛的作用。本研究表明，解淀粉芽孢杆菌FZB42的主要抗真菌剂bacillomycin D的合成受多层调控。bacillomycin D操纵子（bmy）的表达依赖于单个sigma(a)依赖的启动子P-bmy，并在其天然宿主中受到小调节蛋白DegQ的支持。bmy的完全转录激活需要全球调节因子DegU和ComA。DegU保留了一个关键的作用，因为它直接结合到位于芽孢杆菌素D启动子上游的两个位点。此外，DegU和功能未知的跨膜蛋白YczE都作用于较晚水平的基因表达，以迄今未知的方式发挥其转录后作用。
Environmental strain Bacillus amyloliquefaciens FZB42 differs from the domesticated model organism of the same genus, Bacillus subtilis 168, in its ability to promote plant growth and suppress plant-pathogenic organisms present in the rhizosphere. This behavior is exerted mainly through the production of several nonribosomal cyclic lipopeptides and polyketides, which exhibit a broad range of action against phytopathogenic bacteria, fungi, and nematodes. Here, we provide evidence that the synthesis of the main antifungal agent of B. amyloliquefaciens FZB42, bacillomycin D, is regulated in multiple layers. Expression of the bacillomycin D operon (bmy) is dependent on a single sigma(A)-dependent promoter, P-bmy and is favored in its natural host by the small regulatory protein DegQ. The global regulators DegU and ComA are required for the full transcriptional activation of bmy. DegU retains a key role since it binds directly to two sites located upstream of the bacillomycin D promoter. Moreover, both DegU and a transmembrane protein of unknown function, YczE, act on a later level of gene expression, exerting their posttranscriptional effects in a hitherto-unknown manner.