Global position and recruitment of HATs and HDACs in the yeast genome
Global position and recruitment of HATs and HDACs in the yeast genome
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DOI：
10.1016/j.molcel.2004.09.021
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发表时间：
2004-10-22
期刊：
影响因子：
16
通讯作者：
Young, RA
中科院分区：
文献类型：
--
作者：
Robert, F;Pokholok, DK;Young, RA
染色质调节因子在基因表达调控和染色体维持中起着重要作用，但大多数这些调节因子在基因组中的作用区域尚未确定。我们研究了在酿酒酵母中编码的四种不同的染色质调节因子在全基因组中的占有率。结果表明，组蛋白乙酰转移酶Gcn5和Esa1都是活性蛋白编码基因的启动子。相反，组蛋白脱乙酰酶Hst1和Rpd3被招募到与不同细胞功能相关的特定基因集。我们的结果为组蛋白乙酰转移酶和组蛋白去乙酰基酶与酵母基因组的关联提供了新的见解，并结合以前的研究，提出了这些染色质调节因子是如何招募到基因组的特定区域的。
Chromatin regulators play fundamental roles in the regulation of gene expression and chromosome maintenance, but the regions of the genome where most of these regulators function has not been established. We explored the genome-wide occupancy of four different chromatin regulators encoded in Saccharomyces cerevisiae. The results reveal that the histone acetyltransferases Gcn5 and Esa1 are both generally recruited to the promoters of active protein-coding genes. In contrast, the histone deacetylases Hst1 and Rpd3 are recruited to specific sets of genes associated with distinct cellular functions. Our results provide new insights into the association of histone acetyltransferases and histone deacetylases with the yeast genome, and together with previous studies, suggest how these chromatin regulators are recruited to specific regions of the genome.