Microarray analysis reveals age-related differences in gene expression during the development of osteoarthritis in mice.
Microarray analysis reveals age-related differences in gene expression during the development of osteoarthritis in mice.
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DOI：
10.1002/art.33388
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发表时间：
2012-03
影响因子：
--
通讯作者：
Fetrow, Jacquelyn S.
中科院分区：
文献类型：
--
作者：
Loeser, Richard F.;Olex, Amy L.;McNulty, Margaret A.;Carlson, Cathy S.;Callahan, Michael F.;Ferguson, Cristin M.;Chou, Jeff;Leng, Xiaoyan;Fetrow, Jacquelyn S.
更好地了解年龄对骨关节炎（OA）发展的影响。内侧半月板（DMM）的手术失稳用于在12周龄和12月龄雄性C57/BL6小鼠中建立OA模型。对OA严重程度进行组织学评价。用于微阵列和实时PCR的RNA从关节组织中分离，所述关节组织从关节的内侧收集，包括软骨、半月板、软骨下骨和具有滑膜的关节囊。计算分析用于识别基因表达模式，免疫组织化学用于评估所选蛋白质的组织分布。OA在老年小鼠中比年轻小鼠更严重。在两个年龄组中，只有55个基因显示出与DMM诱导的OA相似的表达，而493个基因显示出差异表达，大多数在老年小鼠中表达增加。相似表达基因的功能类别包括细胞外基质和细胞粘附相关基因;差异表达基因包括肌肉结构和发育以及免疫球蛋白结构域基因。在假手术对照关节表达的比较显示，与年龄相关的基质基因表达的减少和免疫和防御反应基因的增加。IL-33存在于多个关节组织细胞中，而CCL 21更多地定位于软骨细胞和软骨细胞。骨膜蛋白存在于软骨和半月板的细胞外基质中。年龄影响关节组织中基因表达的基本模式和对关节炎诱导的OA的反应。检查软骨以外的关节组织发现了新的基因和蛋白质，这些基因和蛋白质在OA中很重要。
To better understand the contribution of age to the development of osteoarthritis (OA). Surgical destabilization of the medial meniscus (DMM) was used to model OA in 12 week-old and 12 month-old male C57/BL6 mice. OA severity was evaluated histologically. RNA used for microarrays and real-time PCR was isolated from joint tissue collected from the medial side of the joint, including cartilage, meniscus, subchondral bone, and joint capsule with synovium. Computational analysis was used to identify patterns of gene expression and immunohistochemistry to evaluate tissue distribution of selected proteins. OA was more severe in older mice than young. Only 55 genes showed a similar expression with DMM-induced OA in the two age groups while 493 genes showed differential expression, the majority having increased expression in older mice. Functional categories for similarly expressed genes included extracellular matrix and cell adhesion related genes; differentially expressed genes included muscle structure and development and immunoglobulin domain genes. Comparison of expression in the sham control joints revealed an age-related decrease in matrix gene expression and an increase in immune and defense response genes. IL-33 was present in multiple joint tissue cells while CCL21 was more localized to chondrocytes and meniscal cells. Periostin was found in the extracellular matrix of cartilage and meniscus. Age affects both the basal pattern of gene expression in joint tissues and the response to surgically-induced OA. Examining tissue from the joint beyond only cartilage revealed novel genes and proteins that would be important to consider in OA.
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影响因子：
--
作者：
Lawrence, Reva C.;Felson, David T.;Wolfe, Frederick
通讯作者：
Wolfe, Frederick
人类关节软骨细胞产生IL-7并对IL-7作出反应，而基质金属蛋白酶13的产生增加。
影响因子：
4.9
作者：
Long, David;Blake, Simon;Song, Xiao-Yu;Lark, Michael;Loeser, Richard F.
通讯作者：
Loeser, Richard F.
影响因子：
--
作者：
Aigner, Thomas;Fundel, Katrin;Bartnik, Eckart
通讯作者：
Bartnik, Eckart
影响因子：
--
作者：
Glasson, SS;Askew, R;Morris, EA
通讯作者：
Morris, EA
DOI：
10.1073/pnas.0610024104
发表时间：
2007-01-30
影响因子：
11.1
作者：
Mirotsou, Maria;Zhang, Zhongyan;Dzau, Victor
通讯作者：
Dzau, Victor