Weipixiao ameliorates gastric precancerous lesions in a rat's model by regulating GSK3β and C-myc
Weipixiao ameliorates gastric precancerous lesions in a rat's model by regulating GSK3β and C-myc
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DOI：
10.1016/s0254-6272(18)30909-9
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发表时间：
2018-10
影响因子：
2.6
通讯作者：
Zeng Jinhao;Hua-feng Pan;Guo Jing;Daoyin Gong;Tiantian Cai;Xiaodong Chen;Z. Yi;Fengming You;Long-hui Chen;Ziming Zhao;Liang Chao
中科院分区：
文献类型：
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作者：
Zeng Jinhao;Hua-feng Pan;Guo Jing;Daoyin Gong;Tiantian Cai;Xiaodong Chen;Z. Yi;Fengming You;Long-hui Chen;Ziming Zhao;Liang Chao
目的探讨胃脾消(WPX)在改善胃癌前病变(GPL)大鼠模型中的作用机制。方法采用高效液相色谱法对WPX制剂的化学成分进行鉴定。 Sprague-Dawley大鼠随机分为对照组、模型组、维生素酶组、WPX高剂量组(H-WPX)、WPX中剂量组(M-WPX)和WPX低剂量组(L-WPX)。造模后给大鼠灌胃WPX或维生素酶，连续10周。分别通过实时定量逆转录聚合酶链式反应（RT-qPCR）和免疫组织化学评估GSK3β、C-myc、Cylin E的基因和蛋白表达。结果SWPX可以有效减轻大鼠“非进行性GPL”的病理改变。正如预期的那样，模型大鼠中 C-myc 和 Cylin E 的 mRNA 和蛋白水平上调，而 GSK3β 表达下调（P < 0.01）。 WPX治疗，特别是低剂量时，可显着下调GPL大鼠C-myc mRNA和蛋白水平，并可导致GSK3β mRNA和蛋白水平显着上调（P<0.05）。然而，在WPX处理的大鼠中没有观察到显着的变化。 结论我们的研究结果表明，WPX介导的GPL病理改变的减弱可能是由于其对GSK3β和C-myc表达的调节作用，以及Wnt/GSK3β通路的失调。
OBJECTIVETo investigate the mechanism underlying the action of Weipixiao (WPX) in a rat's model with ameliorating gastric precancerous lesions (GPL).METHODSHPLC analysis was performed to identify the chemical constituents of WPX preparation. Sprague- Dawley rats were randomly assigned into control group, model group, vitacoenzyme group, high-dose WPX group (H-WPX), medium-dose WPX group (M-WPX) and low-dose WPX group (L-WPX). After modeling, the treated rats were administrated WPX or vitacoenzyme intragastrically for consecutive 10 weeks. Gene and protein expressions of GSK3β, C-myc, Cylin E were evaluated by quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) and immunohistochemistry, respectively.RESULTSWPX could efficiently attenuate the pathological alterations of “non-progressive GPL” in rats. As expected, mRNA and protein levels of C-myc and Cylin E were up-regulated in model rats, while GSK3β expression down-regulated (P< 0.01). WPX treatment, especially at low dose, could significantly down-regulate the mRNA as well as protein levels of C-myc, and could lead to remarkable up-regulation of mRNA and protein levels of GSK3β in GPL rats (P< 0.05). However, no significant changes were observed in WPX-treated rats.CONCLUSIONOur findings suggested that WPX-mediated attenuation of GPL pathological alterations might be due to its regulatory effect on the expressions of GSK3β and C-myc, and on the dysregulation of Wnt/GSK3β pathway.