Thermoforming of tracheal cartilage: viability, shape change, and mechanical behavior.
Thermoforming of tracheal cartilage: viability, shape change, and mechanical behavior.
复制标题
DOI：
10.1002/lsm.20666
复制
发表时间：
2008-10
影响因子：
2.4
通讯作者：
Wong, Brian J. F.
中科院分区：
文献类型：
--
作者：
Chae, Yongseok;Protsenko, Dmitriy;Holden, Paul K.;Chlebicki, Cara;Wong, Brian J. F.
外伤、紧急气管切开术和长时间插管是气管严重变形和狭窄的常见原因。激光技术可用于通过微创方法重塑气管软骨。本研究的目的是确定：（1）分别使用加热盐水浴浸泡和激光照射，气管软骨形状变化对温度和激光剂量测定的依赖性，（2）温度对软骨机械行为的影响，以及（3）组织活力作为激光剂量测定的函数。将离体兔气管软骨标本弯曲并固定在圆柱体（6 毫米）周围，然后浸入盐水浴（45 和 72°C）中 5-100 秒。在单独的实验中，用二极管激光器（λ = 1.45 μm，220–400 J/cm2）照射气管标本。然后使用机械分析来确定辐射后的拉伸弹性模量。采用荧光活力测定结合激光扫描共聚焦显微镜（LSCM）对热损伤区域进行成像和识别。确定了形状变化过渡区，在盐水加热浴中温度为 62 至 66°C 之间，激光照射的功率密度高于 350 J/cm2（峰值温度为 65±10°C）。在这些区域之上，弹性模量（8.2±4 MPa）高于较低温度下的弹性模量（4.5±3 MPa）。 LSCM 发现激光照射区域内存活的软骨细胞显着损失。我们的结果表明，激光照射会导致机械性能发生变化，并进一步证明，使用现有的激光和剂量测定参数，兔气管弹性软骨组织的显着热损伤与临床相关的形状变化同时发生。
Trauma, emergent tracheostomy, and prolonged intubation are common causes of severe deformation and narrowing of the trachea. Laser technology may be used to reshape tracheal cartilage using minimally invasive methods. The objectives of this study were to determine: (1) the dependence of tracheal cartilage shape change on temperature and laser dosimetry using heated saline bath immersion and laser irradiation, respectively, (2) the effect of temperature on the mechanical behavior of cartilage, and (3) tissue viability as a function of laser dosimetry. Ex vivo rabbit trachea cartilage specimens were bent and secured around a cylinder (6 mm), and then immersed in a saline bath (45 and 72°C) for 5– 100 seconds. In separate experiments, tracheal specimens were irradiated with a diode laser (λ = 1.45 μm, 220–400 J/cm2). Mechanical analysis was then used to determine the elastic modulus in tension after irradiation. Fluorescent viability assays combined with laser scanning confocal microscopy (LSCM) were employed to image and identify thermal injury regions. Shape change transition zones, between 62 and 66°C in the saline heating bath and above power densities of 350 J/cm2 (peak temperatures 65±10°C) for laser irradiation were identified. Above these zones, the elastic moduli were higher (8.2±4 MPa) than at lower temperatures (4.5±3 MPa). LSCM identified significant loss of viable chondrocytes within the laser-irradiation zones. Our results indicate a change in mechanical properties occurs with laser irradiation and further demonstrates that significant thermal damage is concurrent with clinically relevant shape change in the elastic cartilage tissues of the rabbit trachea using the present laser and dosimetry parameters.
登录
查看更多内容
影响因子：
3.6
作者：
PENN, RB;WOLFSON, MR;SHAFFER, TH
通讯作者：
SHAFFER, TH
影响因子：
2.4
作者：
Bagratashvili, VN;Sobol, EN;Howdle, SM
通讯作者：
Howdle, SM
影响因子：
3.3
作者：
HABIB, RH;CHALKER, RB;JACKSON, AC
通讯作者：
JACKSON, AC
影响因子：
3.3
作者：
Ramchandani, R;Shen, X;Tepper, RS
通讯作者：
Tepper, RS
DOI：
10.1053/j.semtcvs.2004.08.006
发表时间：
2004-01-01
影响因子：
2.5
作者：
Jaquiss, Robert D B
通讯作者：
Jaquiss, Robert D B