Protein adsorption and materials biocompatibility: A tutorial review and suggested hypotheses
Protein adsorption and materials biocompatibility: A tutorial review and suggested hypotheses
复制标题
DOI：
10.1007/3-540-16422-7_6
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
J. Andrade;V. Hlady
中科院分区：
文献类型：
--
作者：
J. Andrade;V. Hlady
综述了蛋白质在固-液界面上的吸附，包括蛋白质的结构和蛋白质吸附的原理。讨论了基于吸附的生物相容性假设和关联，包括表面电荷、界面能量学、钝化、抗蛋白质表面以及吸附的免疫球蛋白和补体的作用。讨论了研究蛋白质吸附的新方法，包括全内反射技术(吸光度、荧光和拉曼)和椭偏法。给出了蛋白质吸附的定性“经验法则”。
A comprehensive review of protein adsorption at solid-liquid interfaces is presented, including a brief review of protein structure and the principles of protein adsorption. Adsorption-based biocompatibility hypotheses and correlations are discussed, including surface charge, interface energetics, passivation, protein-resistant surfaces, and the role of adsorbed immunoglobulins and complement. New methods for the study of protein adsorption are discussed, including total internal reflection techniques (absorbance, fluorescence, and Raman) and ellipsometry. Qualitative “rules of thumb” of protein adsorption are also presented.