Propidium iodide labeling of nanoparticles as a novel tool for the quantification of cellular binding and uptake
Propidium iodide labeling of nanoparticles as a novel tool for the quantification of cellular binding and uptake
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DOI：
10.1016/j.nano.2010.12.007
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发表时间：
2011-08-01
影响因子：
5.4
通讯作者：
Daum, Nicole
中科院分区：
文献类型：
--
作者：
Neumeyer, Andrea;Bukowski, Mirko;Daum, Nicole
由于纳米粒子在药物输送方面是很有前途的工具，量化它们的细胞结合和摄取是一个新出现的问题。因此，我们研究了不同大小和表面修饰的罗丹明B异硫氰酸酯标记的二氧化硅纳米颗粒在Caco-2细胞中的摄取。流式细胞仪研究显示，未经修饰的纳米颗粒(50和77 nm)与大小和时间相关，而较大的颗粒(94 Nm)和聚乙二醇改性的纳米颗粒没有显示出细胞相互作用。第二种方法处理带有吸附的碘化丙啶(PI)的颗粒，以区分内在化和吸附的纳米颗粒。这些颗粒只有在与细胞内的核酸相关时才会发出荧光信号，而吸附在细胞外表面的颗粒则不会被检测到。PI标记的纳米颗粒(21 Nm)显示出时间依赖性的摄取，在细胞质中有信号，但在细胞核中较少。这些新的PI标记纳米颗粒与流式细胞仪相结合是定量纳米颗粒摄取的创新工具。来自临床编辑：罗丹明B异硫氰酸酯标记的不同尺寸和表面修饰的二氧化硅纳米颗粒关于它们的细胞摄取的研究。含有碘化丙胺的颗粒只有在与核酸结合时才会发出荧光信号，并有助于检测颗粒的内化。这些新的纳米颗粒与流式细胞术相结合，是量化纳米颗粒摄取的创新工具。(C)2011 Elsevier Inc.保留所有权利。
Because nanoparticles are promising tools in drug delivery, quantification of their cellular binding and uptake is an emerging question. Therefore, rhodamine B isothiocyanate-labeled silica nanoparticles with different sizes and surface modifications were investigated concerning their uptake in Caco-2 cells. Flow cytometry studies exhibited a size- and time-dependent association for unmodified nanoparticles (50 and 77 nm), whereas larger particles (94 nm) and polyethylene glycol-modified nanoparticles showed no cellular interaction. A second approach dealt with particles with adsorbed propidium iodide (PI) to distinguish between internalized and adsorbed nanoparticles. These particles only give a fluorescence signal when associated with nucleic acids inside the cell, whereas particles adsorbed to the outer cell surface are not detected. PI-labeled nanoparticles (21 nm) showed a time-dependent uptake, exhibiting a signal in the cytoplasm but less in the nucleus. These novel PI-labeled nanoparticles in combination with flow cytometry are innovative tools for the quantification of nanoparticulate uptake.From the Clinical Editor: Rhodamine B isothiocyanate-labeled silica nanoparticles with different sizes and surface modifications were investigated concerning their cellular uptake. Propidium iodide containing particles only give a fluorescence signal when associated with nucleic acids and are useful in detecting internalization of the particles. These novel nanoparticles in combination with flow cytometry are innovative tools for the quantification of nanoparticulate uptake. (C) 2011 Elsevier Inc. All rights reserved.