Regulation of murine class I genes by interferons is controlled by regions located both 5' and 3' to the transcription initiation site.
Regulation of murine class I genes by interferons is controlled by regions located both 5' and 3' to the transcription initiation site.
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干扰素对小鼠 I 类基因的调节是由位于转录起始位点 5 和 3 的区域控制的。
DOI：
10.1073/pnas.84.10.3380
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发表时间：
1987
影响因子：
11.1
通讯作者：
Stroynowski,I
中科院分区：
文献类型：
--
作者：
Korber,B;Hood,L;Stroynowski,I
干扰素调节大量哺乳动物基因的表达，包括主要组织相容性抗原基因。为了研究干扰素作用的机制，我们分析了小鼠H-2Ld和H-2Dd DNA序列控制干扰素应答的能力。结果表明，干扰素调节I类基因的表达是复杂的，涉及至少两种机制，依赖于位于上游和下游的转录起始位点的I类序列。在转染的小鼠L细胞中，这两个区域都是完全增强I类基因表达所必需的，主要部分的反应由位于转录起始位点3'端的序列控制。5'区编码的反应的精细作图分析还表明，重组α和γ干扰素可能通过使用不同的顺式作用调节序列对I类基因表达发挥作用。
Interferons regulate the expression of a large number of mammalian genes, including the major histocompatibility antigen genes. To investigate the mechanisms involved in interferon action, we have analyzed the ability of murine H-2Ld and H-2Dd DNA sequences to control the responses to interferon. The results indicate that interferon regulation of class I gene expression is complex and involves at least two mechanisms that are dependent on class I sequences located upstream and downstream to the transcription initiation site. In transfected mouse L cells, both of these regions are required for full enhancement of class I gene expression, with the major portion of the response controlled by the sequences located 3' to the transcription initiation site. The fine-mapping analysis of the 5' region-encoded response also suggests that recombinant alpha and gamma interferons may exert their effects on class I gene expression by using different cis-acting regulatory sequences.