The Neurospora aab-1 gene encodes a CCAAT binding protein homologous to yeast HAP5.
The Neurospora aab-1 gene encodes a CCAAT binding protein homologous to yeast HAP5.
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脉孢菌 aab-1 基因编码与酵母 HAP5 同源的 CCAAT 结合蛋白。
作者：
H. Chen;J. Crabb;J. Kinsey
am（谷氨酸脱氢酶）基因的表达依赖于两个上游激活序列，称为URSam（α）和URSam（β）。异聚体核蛋白Am α结合蛋白（AAB）特异性结合URSam（α）元件内的CCAAT盒。AAB似乎由三个部分组成。我们使用多克隆抗血清对高度纯化的AAB 1亚基分离的部分aab-1的cDNA克隆，然后用于分离全长cDNA和基因组克隆。全长cDNA有可能编码一个272个氨基酸的蛋白质，计算分子量为30 kD。通过对AAB 1蛋白的Edman分析获得的氨基酸序列证实aab-1基因已被克隆。AAB-1与酵母的HAP 5蛋白和大鼠的CBF-C蛋白具有相似性。这些蛋白质中的每一种都是其各自异聚体CCAAT结合蛋白的必需亚基。aab 1基因定位于粗糙脉孢菌的连锁群III上靠近trp-1位点。破坏aab-1基因会导致对生长和发育的多效性影响，以及谷氨酸脱氢酶水平降低50%。用克隆的aab-1基因的基因组拷贝转化aab-1破坏突变株挽救了与aab-1突变相关的所有表型改变。
The expression of the am (glutamate dehydrogenase) gene is dependent upon two upstream activating sequences, designated URSam(alpha) and URSam(beta). A heteromeric nuclear protein Am Alpha Binding protein (AAB) binds specifically to a CCAAT box within the URSam(alpha) element. AAB appears to be composed of three components. We used polyclonal antiserum raised against the highly purified AAB1 subunit to isolate a partial aab-1 cDNA clone, which was then used to isolate a full-length cDNA and a genomic clone. The full-length cDNA has the potential to encode a 272 amino acid protein with a calculated molecular weight of 30 kD. Amino acid sequence obtained by Edman analysis of the AAB1 protein confirmed that the aab-1 gene had been cloned. AAB-1 shows similarity to the HAP5 protein of yeast and the CBF-C protein of rat. Each of these proteins is an essential subunit of their respective heteromeric CCAAT binding proteins. The aab1 gene maps on linkage group III of Neurospora crassa near the trp-1 locus. Disruption of the aab-1 gene results in pleiotropic effects on growth and development as well as a 50% reduction in glutamate dehydrogenase levels. Transformation of the aab-1 disruption mutant strain with the cloned genomic copy of the aab-1 gene rescued all of the phenotypic alterations associated with the aab-1 mutation.
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DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Crabb,JW;Johnson,CM;Carr,SA;Armes,LG;Saari,JC
通讯作者：
Saari,JC
DOI：
10.1073/pnas.89.5.1929
发表时间：
1992-03-01
影响因子：
11.1
作者：
MAKOS, M;NELKIN, BD;BAYLIN, SB
通讯作者：
BAYLIN, SB
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DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sachs,MS;David,M;Werner,S;RajBhandary,UL
通讯作者：
RajBhandary,UL
DOI：
10.1016/s0021-9258(18)45730-4
发表时间：
1990-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Vuorio;S. Maity;B. Crombrugghe
通讯作者：
T. Vuorio;S. Maity;B. Crombrugghe
影响因子：
10.5
作者：
FORSBURG, SL;GUARENTE, L
通讯作者：
GUARENTE, L