Bioinspired peptide adhesion on Ti implants alleviates wear particle-induced inflammation and improves interfacial osteogenesis
Bioinspired peptide adhesion on Ti implants alleviates wear particle-induced inflammation and improves interfacial osteogenesis
复制标题
钛种植体上的仿生肽粘附可减轻磨损颗粒引起的炎症并改善界面成骨
DOI：
10.1016/j.jcis.2021.07.079
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发表时间：
2021-07-28
影响因子：
9.9
通讯作者：
Geng, Dechun
中科院分区：
文献类型：
--
作者：
Guo, Xiaobin;Bai, Jiaxiang;Geng, Dechun
在炎症性种植体周围微环境中，巨噬细胞过度极化至促炎性 M1 表型可触发炎症细胞因子的分泌，从而促进骨吸收并阻碍种植体周围的成骨。这一过程的直接后果是假体植入物因无菌性松动而失败。为了扭转炎症微环境并防止假体松动，采用受贻贝粘附启发的表面策略对具有整合素结合能力的钛植入物进行生物工程。在我们的设计中，合成了具有四价 3,4-二羟基-L-苯丙氨酸 (DOPA) 和 Arg-Gly-Asp (RGD) 序列的受贻贝启发的儿茶素肽。该肽可以通过贻贝粘附机制轻松锚定在医用钛材料的表面。我们发现，肽修饰的钛种植体可以在磨损颗粒模型中有效抑制种植体周围炎症，并可以通过干扰整合素-α（2）β（1）和整合素-α v beta（3）促进巨噬细胞极化为促愈合M2表型。此外，即使在炎症条件下，肽涂层也能增加成骨细胞的粘附并促进钛植入物的成骨。这项工作表明，这种仿生儿茶酚整合素结合肽可以为医疗假体的表面生物工程提供简便的策略，改善炎症条件下的界面成骨，这可能对预防假体松动和改善临床结果做出巨大贡献。 (C) 2021 Elsevier Inc. 保留所有权利。
In the inflammatory peri-implant microenvironment, excessive polarization of macrophages to the proinflammatory M1 phenotype can trigger the secretion of inflammatory cytokines, which promote bone resorption and impede osteogenesis around implants. The direct consequence of this process is the failure of prosthetic implants due to aseptic loosening. To reverse the inflammatory microenvironment and prevent prosthesis loosening, a mussel adhesion-inspired surface strategy was used for bioengineering of titanium implants with integrin-binding ability. In our design, a mussel-inspired catecholic peptide with tetravalent 3,4-dihydroxy-L-phenylalanine (DOPA) and Arg-Gly-Asp (RGD) sequences was synthesized. The peptide can easily anchor to the surface of medical titanium materials through a mussel adhesive mechanism. We found that peptide-decorated titanium implants could effectively inhibit peri-implant inflammation in a wear particle model and could promote the polarization of macrophages to a pro-healing M2 phenotype by interfering with integrin-alpha(2)beta(1) and integrin-alpha v beta(3). Moreover, the peptide coating increased the adherence of osteoblasts and promoted osteogenesis on titanium implants even under inflammatory conditions. This work suggested that this biomimetic catecholic integrin-binding peptide can provide facile tactics for surface bioengineering of medical prostheses with improved interfacial osteogenesis under inflammatory conditions, which might contribute greatly to the prevention of pros- thesis loosening and the improvement of clinical outcomes. (C) 2021 Elsevier Inc. All rights reserved.