An advanced hybrid medium optimization strategy for the enhanced productivity of lutein in Chlorella minutissima
An advanced hybrid medium optimization strategy for the enhanced productivity of lutein in Chlorella minutissima
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DOI：
10.1016/j.algal.2014.11.010
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发表时间：
2015-01-01
影响因子：
5.1
通讯作者：
Sen, Ramkrishna
中科院分区：
文献类型：
--
作者：
Dineshkumar, R.;Dhanarajan, Gunaseelan;Sen, Ramkrishna
微藻中叶黄素的生物合成受培养基成分的影响，培养基成分可以增加叶黄素代谢途径中关键酶的浓度，也可以作为叶黄素代谢途径中相关酶的辅助因子。采用Plackett-Burman设计筛选Bold's基础培养基（BBM）组分，并结合人工神经网络建模和粒子群优化（ANN-PSO）的培养基优化策略，提高小球藻叶黄素的产量。在这两步混合优化奋进中，Plackett-Burman分析帮助选择NaNO 3，KH 2 PO 4，MnCl 2中心点4 H（2）O和CuSO 4中心点5 H（2）O作为关键组分，随后通过ANN-PSO技术进行优化。在实验验证中，优化的培养基导致叶黄素生产率比BBM高三倍。采用优化的培养基和适宜的工艺条件，在2L气升式光生物反应器中培养小球藻，叶黄素产量最高可达3.45 ± 0.07 mg L-1 d（-1）。因此，混合优化方法有助于将叶黄素产率提高约187%，这显著高于文献中报道的相关值。据我们所知，这是第一个报告的应用程序的集成优化策略，提高叶黄素生产力的小球藻作为一个模式光自养生物。(C)2014爱思唯尔有限公司版权所有。
The biosynthesis of lutein in microalgae is critically influenced by the media components, which either increase the key enzyme concentrations or act as cofactors for the enzymes involved in lutein metabolic pathway. In this study, an advanced medium optimization strategy, involving Plackett-Burman design for screening the Bold's Basal Medium(BBM) components, followed by artificial neural network modeling and particle swarmo-ptimization (ANN-PSO), was implemented for improving the productivity of lutein in Chlorella minutissima. In this two-step hybrid optimization endeavor, Plackett-Burman analysis helped select NaNO3, KH2PO4, MnCl2 center dot 4H(2)O and CuSO4 center dot 5H(2)O as critical components that were subsequently optimized by ANN-PSO technique. On experimental validation, the optimized medium resulted in three-fold greater lutein productivity, as compared to BBM. Furthermore, the maximum lutein productivity of 3.45 +/- 0.07 mg L-1 d(-1) was obtained upon cultivation of Chlorella minutissima in a 2-L airlift photobioreactor using the optimized medium with appropriate process conditions. Thus, the hybrid optimization approach was instrumental in enhancing lutein productivity by about 187%, which was significantly higher than the relevant values reported in literature. To our knowledge, this is the first report on the application of an integrated optimization strategy for the enhanced lutein productivity in Chlorella minutissima as a model photo-autotrophic organism. (C) 2014 Elsevier B.V. All rights reserved.