Synergistic Angiogenesis Promoting Effects of Extracellular Matrix Scaffolds and Adipose-Derived Stem Cells During Wound Repair
Synergistic Angiogenesis Promoting Effects of Extracellular Matrix Scaffolds and Adipose-Derived Stem Cells During Wound Repair
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DOI：
10.1089/ten.tea.2010.0331
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发表时间：
2011-03-01
影响因子：
4.1
通讯作者：
Jin, Yan
中科院分区：
文献类型：
--
作者：
Liu, Shiyu;Zhang, Hongmei;Jin, Yan
血管化速度慢被认为是用于伤口愈合的支架的主要缺点之一。已经做出了一些努力，包括细胞和脱细胞技术，以诱导支架中的血管生长。然而，迄今为止，还没有建立诱导血管生长的技术。本研究的目的是通过在支架上接种脂肪干细胞（ADSCs）来促进支架的血管化能力，并比较不同支架接种ADSCs后的血管化能力。选用两种细胞外基质支架（小肠黏膜下层[SIS]和脱细胞真皮基质[ADM]）和一种复合支架（胶原-硫酸软骨素-透明质酸[Co-CS-HA]）。皮下植入实验表明，SIS和ADM的血管化能力均大于Co-CS-HA。接种在SIS和ADM中的ADSC比接种在Co-CS-HA中的ADSC分泌更大量的血管内皮生长因子。在小鼠皮肤损伤模型中，与未接种支架相比，接种ADSC的支架增强了血管生成和伤口愈合率。ADSC-SIS和ADSC-ADM的血管化能力均高于ADSC-Co-CS-HA。总之，这些结果表明，脂肪干细胞可以作为一种细胞来源，以促进支架的血管化能力。ADSC种植支架的血管化能力受到支架本身的血管化能力及其对ADSC血管生成潜力的影响;细胞外基质支架和ADSC的组合表现出协同的血管生成促进作用。
Slow vascularization rate is considered one of the main drawbacks of scaffolds used in wound healing. Several efforts, including cellular and acellular technologies, have been made to induce vascular growth in scaffolds. However, thus far, there is no established technology for inducing vascular growth. The aim of this study was to promote the vascularization capacities of scaffolds by seeding adipose-derived stem cells (ADSCs) on them and to compare the vascularization capacities of different scaffolds seeded with ADSCs. Two kinds of extracellular matrix scaffolds (small intestinal submucosa [SIS] and acellular dermal matrix [ADM]) and a kind of composite scaffold (collagen-chondroitin sulfate-hyaluronic acid [Co-CS-HA]) were selected. Subcutaneous implantation analysis showed that the vascularization capacity of SIS and ADM was greater than that of Co-CS-HA. ADSCs seeded in SIS and ADM secreted greater amounts of vascular endothelial growth factor than those seeded in Co-CS-HA. In a murine skin injury model, ADSC-seeded scaffolds enhanced the angiogenesis and wound healing rate compared with the nonseeded scaffolds. Moreover, ADSC-SIS and ADSC-ADM had greater vascularization capacity than that of ADSC-Co-CS-HA. Taken together, these results suggest that ADSCs could be used as a cell source to promote the vascularization capacities of scaffolds. The vascularization capacities of ADSC-seeded scaffolds were influenced by both the vascularization capacities of the scaffolds themselves and their effects on the angiogenic potential of ADSCs; the combination of extracellular matrix scaffolds and ADSCs exhibited synergistic angiogenesis promoting effects.