Association of Frabin with the Actin Cytoskeleton Is Essential for Microspike Formation through Activation of Cdc42 Small G Protein*
Association of Frabin with the Actin Cytoskeleton Is Essential for Microspike Formation through Activation of Cdc42 Small G Protein*
复制标题
Frabin 与肌动蛋白细胞骨架的结合对于通过激活 Cdc42 小 G 蛋白形成微刺至关重要*
DOI：
10.1074/jbc.274.36.25197
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Y. Takai
中科院分区：
文献类型：
--
作者：
M. Umikawa;H. Obaishi;H. Nakanishi;Keiko Satoh;Kenichi Takahashi;Ikuko Hotta;Y. Matsuura;Y. Takai
我们最近分离出一种新的肌动蛋白亲和结合蛋白，命名为frabin。Frabin从N端到C端依次具有一个肌动蛋白毒力结合结构域（ABD）、一个Db 1同源结构域（DHD）、与DHD相邻的第一普列克底物蛋白同源结构域（PHD）、一个富含半胱氨酸的结构域（CRD）和第二PHD。全长frabin诱导微刺形成和c-Jun N-末端激酶（JNK）活化。我们发现含有DHD和第一PHD的frabin片段刺激Cdc 42 Hs小G蛋白的鸟嘌呤核苷酸交换，但不刺激RhoA或Rac 1小G蛋白的鸟嘌呤核苷酸交换。然而，该片段的frabin没有诱导微刺形成，和ABD是额外必要的微刺形成。具有ABD的Frabin与肌动蛋白细胞骨架相关，而缺乏ABD的frabin则弥漫分布在细胞质中。相反，ABD不是JNK激活所必需的，但CRD和第二PHD对于这种激活是额外必需的。这些结果表明，该协会的frabin与肌动蛋白细胞骨架是必不可少的微刺形成，但不是JNK激活，并参与不同领域的frabin通过Cdc 42激活微刺形成和JNK激活。
We have recently isolated a novel actin filament-binding protein, named frabin. Frabin has one actin filament-binding domain (ABD), one Dbl homology domain (DHD), first pleckstrin homology domains (PHD) adjacent to DHD, one cysteine rich-domain (CRD), and second PHD from the N terminus to the C terminus in this order. Full-length frabin induces microspike formation and c-Jun N-terminal kinase (JNK) activation. We found here that the fragment of frabin containing DHD and first PHD stimulated guanine nucleotide exchange of Cdc42Hs small G protein, but not that of RhoA or Rac1 small G protein. However, this fragment of frabin did not induce microspike formation, and ABD was additionally necessary for microspike formation. Frabin having ABD was associated with the actin cytoskeleton, whereas frabin lacking ABD was diffusely distributed in the cytoplasm. In contrast, ABD was not necessary for JNK activation but CRD and second PHD were additionally necessary for this activation. These results indicate that the association of frabin with the actin cytoskeleton is essential for microspike formation but not for JNK activation and that different domains of frabin are involved in microspike formation and JNK activation through Cdc42 activation.
影响因子：
2.9
作者：
GUAN, KL;DIXON, JE
通讯作者：
DIXON, JE