TESTICULAR MACROPHAGES - ISOLATION, CHARACTERIZATION AND HORMONAL RESPONSIVENESS
TESTICULAR MACROPHAGES - ISOLATION, CHARACTERIZATION AND HORMONAL RESPONSIVENESS
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DOI：
10.1095/biolreprod29.5.1319
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发表时间：
1983-01-01
影响因子：
3.6
通讯作者：
HUTSON, JC
中科院分区：
文献类型：
--
作者：
YEE, JB;HUTSON, JC
用胰蛋白酶处理从大鼠睾丸中分离巨噬细胞，并使用差异附着技术在培养物中建立巨噬细胞。将细胞在32 ℃的培养基199中培养。C.然后，细胞的特征在于它们表达传统免疫功能的能力以及在各种激素的调节下分泌乳酸的能力。巨噬细胞的活细胞培养物明显获得，因为：细胞强烈染色的非特异性酯酶;他们拥有Fc受体在他们的细胞质膜上;他们能够吞噬3 H-标记的大肠杆菌和碳颗粒;和他们是高度抵抗胰蛋白酶的影响，诱导脱离培养基质。这些培养物没有被Leydig细胞或Sertoli细胞污染，因为它们对3 β-羟基类固醇脱氢酶，不分泌雄激素结合蛋白（ABP）。这些细胞能够通过增加乳酸的分泌以剂量依赖性方式响应FSH。用1 μ g FSH/ml观察到最大刺激，其导致比对照值增加2.5倍。二丁酰cAMP（dbcAMP）也引起了这些细胞的乳酸产量的剂量相关性增加。促黄体生成激素、胰岛素、睾丸激素或17 β雌二醇对这些细胞的乳酸产生没有类似的影响。腹腔巨噬细胞对FSH或dbcAMP无反应。睾丸巨噬细胞的高度富集的人口显然可以保持在文化和表达传统上归因于巨噬细胞的几个免疫学特征。这些细胞能够以特定的方式对FSH作出反应。
Macrophages were isolated from rat testes with trypsin treatment and established in culture using a differential attachment technique. The cells were maintained in culture in Medium 199 at 32.degree. C. The cells were then characterized for their ability to express traditional immunological function as well as to secrete lactate under the regulation of various hormones. Viable cultures of macrophages evidently were obtained since: the cells stained intensely for nonspecific esterase; they possessed Fc receptors on their cytoplasmic membranes; they were capable of phagocytosing 3H-labeled Escherichia coli and carbon particles; and they were highly resistant to the effects of trypsin to induce detachment from the culture substrate. These cultures were not contaminated with Leydig cells or Sertoli cells since they were negative for 3.beta.-hydroxy-steroid dehydrogenase and did not secrete androgen-binding protein (ABP). These cells were capable of responding to FSH in a dose-dependent manner by increasing the secretion of lactate. Maximal stimulation was observed with 1 .mu.g FSH/ml which resulted in a 2.5-fold increase over control values. Dibutyryl cAMP (dbcAMP) also caused a dose-related increase in lactate production by these cells. Luteinizing hormone, insulin, testosterone or 17.beta.-estradiol had no similar effect on lactate production by these cells. Peritoneal macrophages were not responsive to FSH or dbcAMP. A highly enriched population of testicular macrophages apparently can be maintained in culture and express several immunological characteristics traditionally ascribed to macrophages. These cells were capable of responding to FSH in a specific manner.