Visualization of CD44 and CD133 in Normal Pancreas and Pancreatic Ductal Adenocarcinomas: Non-overlapping Membrane Expression in Cell Populations Positive for Both Markers
Visualization of CD44 and CD133 in Normal Pancreas and Pancreatic Ductal Adenocarcinomas: Non-overlapping Membrane Expression in Cell Populations Positive for Both Markers
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DOI：
10.1369/0022155411398275
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发表时间：
2011-04-01
影响因子：
3.2
通讯作者：
Molven, Anders
中科院分区：
文献类型：
--
作者：
Immervoll, Heike;Hoem, Dag;Molven, Anders
基于CD133或CD44的表达，已分离出胰腺导管腺癌(PDAC)的肿瘤起始细胞。作者的目标是通过使用与细胞分选研究中常用的抗体相同的抗体，使胰腺细胞同时表达这两种细胞表面标记。对51例PDAC患者的正常胰腺组织和病变胰腺组织进行分析。福尔马林固定材料免疫组织化学双重染色检测CD44、CD133的表达，免疫荧光/共聚焦显微镜检测亚细胞蛋白分布。在正常胰腺中，CD44和CD133在中央腺泡区域共表达，但在非重叠的亚细胞室表达。不出所料，CD44主要位于基底侧，而CD133位于根尖/腔内膜上。在慢性炎症/萎缩的胰腺组织和PDAC中也是如此。在某些恶性导管中，CD44位于与CD133表达相邻但从不重叠的顶端细胞膜上。CD44水平与患者的淋巴结状况显著相关。总之，在正常胰腺和肿瘤胰腺中确实存在CD44+/CD133+细胞群。在正常实质中，双阳性细胞优先位于中央腺泡内，这表明这一群体在试图识别PDAC中的肿瘤启动细胞时可能特别感兴趣。本文包含http://www.jhc.org.上的在线补充材料请在线访问这篇文章以查看这些材料。(组织化学细胞化学杂志59：441-455,2011)
Tumor-initiating cells of pancreatic ductal adenocarcinoma (PDAC) have been isolated based on expression of either CD133 or CD44. The authors aimed to visualize pancreatic cells simultaneously expressing both these cell surface markers by employing the same antibodies commonly used in cell-sorting studies. Normal and diseased pancreatic tissue, including 51 PDAC cases, were analyzed. CD44 and CD133 expression was determined by immunohistochemical double staining on formalin-fixed material and subcellular protein distribution evaluated by immunofluorescence/confocal microscopy. In the normal pancreas, CD44 and CD133 were coexpressed in the centroacinar regions but in non-overlapping subcellular compartments. As expected, CD44 was found mainly basolaterally, whereas CD133 was present on the apical/endoluminal membrane. This was also the case in chronically inflamed/atrophic pancreatic tissue and in PDAC. In some malignant ducts, CD44 was found at the apical cell membrane adjacent to but never overlapping with CD133 expression. CD44 level was significantly associated with the patient's lymph node status. In conclusion, a CD44+/CD133+ cell population does exist in the normal and neoplastic pancreas. The preferentially centroacinar localization of the doubly positive cells in the normal parenchyma suggests that this population could be of particular interest in attempts to identify tumor-initiating cells in PDAC. This article contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials. (J Histochem Cytochem 59:441-455, 2011)