Layer-specific mitochondrial diversity across hippocampal CA2 dendrites.
Layer-specific mitochondrial diversity across hippocampal CA2 dendrites.
复制标题
海马 CA2 树突的层特异性线粒体多样性。
DOI：
10.1002/hipo.23512
复制
发表时间：
2023
期刊：
影响因子：
3.5
通讯作者：
Farris,Shannon
中科院分区：
文献类型：
--
作者：
Pannoni,KatyE;Gil,Daniela;Cawley,MikelL;Alsalman,MaydM;Campbell,LoganA;Farris,Shannon
CA2是海马体的一个研究不足的亚区，对社会记忆至关重要。先前的研究确定了线粒体钙单向转运体（MCU）复合物的多种组分选择性地富集在CA2中。MCU复合物调节钙进入线粒体，这反过来又调节线粒体转运和定位到活性突触。我们发现MCU在CA2远端顶端树突中显著富集，正是CA2神经元接收携带社会信息的内嗅皮层输入的地方。此外，与CA 2近端顶树突和邻近的CA 1顶树突中的线粒体相比，CA 2远端树突中的MCU富集的线粒体更大，这在CA 2中用遗传标记的线粒体和电子显微镜证实。MCU过度表达在相邻的CA 1导致的MCU在CA 1的近端树突相比，远端树突的优先定位，在CA 2中没有看到的效果。我们的研究结果表明，线粒体在海马细胞类型和电路的分子和结构上是多样化的，并表明MCU可以在树突内差异定位，可能是为了满足当地的能量需求。
CA2 is an understudied subregion of the hippocampus that is critical for social memory. Previous studies identified multiple components of the mitochondrial calcium uniporter (MCU) complex as selectively enriched in CA2. The MCU complex regulates calcium entry into mitochondria, which in turn regulates mitochondrial transport and localization to active synapses. We found that MCU is strikingly enriched in CA2 distal apical dendrites, precisely where CA2 neurons receive entorhinal cortical input carrying social information. Furthermore, MCU‐enriched mitochondria in CA2 distal dendrites are larger compared to mitochondria in CA2 proximal apical dendrites and neighboring CA1 apical dendrites, which was confirmed in CA2 with genetically labeled mitochondria and electron microscopy. MCU overexpression in neighboring CA1 led to a preferential localization of MCU in the proximal dendrites of CA1 compared to the distal dendrites, an effect not seen in CA2. Our findings demonstrate that mitochondria are molecularly and structurally diverse across hippocampal cell types and circuits, and suggest that MCU can be differentially localized within dendrites, possibly to meet local energy demands.