PROCYANIDIN PRODUCTION BY FAGOPYRUM-ESCULENTUM CALLUS-CULTURE
PROCYANIDIN PRODUCTION BY FAGOPYRUM-ESCULENTUM CALLUS-CULTURE
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DOI：
10.1055/s-2006-961539
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发表时间：
1992-12-01
期刊：
影响因子：
2.7
通讯作者：
PINKAS, M
中科院分区：
文献类型：
--
作者：
MOUMOU, Y;TROTIN, F;PINKAS, M
在B5培养基上生长的荞麦愈伤组织培养物合成原花青素B2（0.6-1.8 mg/g干重）和B2- 3 '-O-没食子酸酯（3.5- 6.0 mg/g干重）。蔗糖是比其他碳水化合物更好的来源，并且中等浓度（3-4%）刺激生长和原花青素合成。而黑暗不是一个限制因素，光诱导更快的增长和原花青素含量增加。用没食子酸处理强烈地刺激原花青素B2- 3 '-O-没食子酸酯的产生，但显著地抑制生长，导致结合方便的生长和增加的没食子酰化二聚体合成（高达45 mg/g干重）的两步培养实验。
Fagopyrum esculentum callus cultures grown on B5 medium synthesize procyanidins B2 (0.6-1.8 mg/g dry wt) and B2-3'-O-gallate (3.5- 6.0 mg/g dry wt). Sucrose is a better source than other carbohydrates and moderate concentrations (3-4%) stimulate both growth and procyanidin synthesis. While darkness was not a limiting factor, light induced faster growth and increased procyanidin contents. Treatment with gallic acid strongly stimulated the procyanidin B2-3'-O-gallate production, but drastically inhibited growth, leading to a two-step-culture experiment combining convenient growth and increased galloylated dimer synthesis (up to 45mg/g dry wt).