Observation of Cell-Substrate Attachment with the Acoustic Microscope
Observation of Cell-Substrate Attachment with the Acoustic Microscope
复制标题
用声学显微镜观察细胞与基底的附着
DOI：
10.1109/t-su.1985.31600
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
J. Hildebrand
中科院分区：
文献类型：
--
作者：
J. Hildebrand
在1.7 GHz下工作的扫描反射声显微镜用于观察活细胞与其基底之间的接触。当声学显微镜透镜聚焦在基底表面上时，细胞-基底附着的位点在声学图像中显示为暗区。定量分析表明，附着位点相对于细胞内的其他区域具有增加的声阻抗。运动细胞的声学图像显示在附着部位的阻抗的空间和时间变化。
Abstmcf-The scanning reflection acoustic microscope operating at 1.7 GHz is used to observe the contacts between living cells and their substrates. When the acoustic microscope lens is focused on the surface of the substrate, sites of cell-substrate attachment appear as darkened areas in the acoustic image. Quantitative analysis indicates that attachment sites have increased acoustic impedance relative to other regions within the cell. Acoustic images of moving cells show spatial and temporal changes in the impedance at the attachment site.