Cryopreservation of human germinal vesicle stage and in vitro matured M II oocytes: influence of cryopreservation media on the survival, fertilization, and early cleavage divisions
Cryopreservation of human germinal vesicle stage and in vitro matured M II oocytes: influence of cryopreservation media on the survival, fertilization, and early cleavage divisions
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DOI：
10.1016/s0015-0282(00)00672-5
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发表时间：
2000-09-01
影响因子：
6.7
通讯作者：
Dhont, M
中科院分区：
文献类型：
--
作者：
Goud, A;Goud, P;Dhont, M
目的：研究低钠冷冻对冻融期和体外成熟人卵母细胞存活和发育的影响。设计：前瞻性实验研究。地点：学术医院生育中心。患者(S)：实验组：卵母细胞冷冻保存在生发泡期(A组，n=63)和生发泡期(C组，n=62)，同时使用常规(A组)或低钠(B组，C组)卵母细胞。对照组：同胞GV期卵母细胞体外成熟(IVM；对照组A，n=；对照组B，n=)。干预组(S)：体外成熟、卵母细胞浆内单精子注射及后续培养。主要观察指标(S)：存活率、成熟率、受精率和卵裂率。结果(S)：A组和B组的解冻后存活率(57.1%)和B组(48.4%)显著低于C组(84.4%)。A组成熟率和卵裂率显著低于对照组，受精率与对照组相似(GVBD：72.2%比90.6%；进展到M II：33.3%比76.6%；卵裂：42.9%比88.2%；受精率：58.3%比69.4%)。B组则无明显差异。在C组中，尽管2个卵母细胞卵裂率略低于B组(47.4%vs.70.2%)，3个卵母细胞卵裂率(3PN：15.8%vs.3.2%)显著高于A组(4.8%)，但与B组(15.6%)相比差异不显著。B组GV期卵母细胞的成熟率和卵裂率显著高于A组(S)。结论：低钠液有利于MⅡ期卵母细胞的体外成熟，明显优于传统的钠基液。(Fertil Steril(R)2000；74：487-93.(C)2000年美国生殖医学学会。)。
Objective: To study the influence of low-sodium cryopreservation media (CPM) on the survival and development of frozen-thawed germinal vesicle (GV) stage and in vitro matured human oocytes.Design: Prospective experimental study.Setting: Academic hospital-based fertility center.Patient(s): Experimental groups: Oocytes cryopreserved at the GV (group A, n = 63 and group B, n = 64) or M II stage (group C, n = 62) With use Of conventional (group A) or low-sodium CPM (groups B and C). Control groups: Sibling GV stage oocytes subjected to in vitro maturation (IVM; control group A, n = 64; control group B, n = 64).Intervention(s): IVM, intracytoplasmic sperm injection and subsequent culture.Main Outcome Measure(s): Rates of survival, maturation, fertilization, and cleavage.Result(s): The postthaw survival was significantly lower in groups A (57.1%) and B (48.4%) compared to C (84.4%). In group A, maturation and cleavage rates were significantly lower, and fertilization rate was similar to controls (GVBD: 72.2% vs. 90.6%; progression to M II: 33.3% vs. 76.6%; cleavage: 42.9% vs. 88.2%; and fertilization: 58.3% vs. 69.4% in group A vs, control group A, respectively). There was no such difference in group B. In group C, despite a slight but significant lowering of the rate of 2 PN and an increase in that of 3 PN (2 PN: 47.4% vs. 70.2% and 3 PN: 15.8% vs. 3.2% in group C vs. total controls, respectively), embryonic cleavage per GV oocyte was significantly higher (25.8%) compared to group A (4.8%) but not to group B (15.6%). The rate of maturation and cleavage per surviving GV oocyte was significantly higher in group B than group A.Conclusion(s): Low-sodium-based CPM is beneficial for in vitro matured M II stage oocytes and is significantly better than the conventional sodium-based media for the GV stage oocytes. (Fertil Steril(R) 2000; 74:487-93. (C) 2000 by American Society for Reproductive Medicine.).