Identification of a plastid-specific ribosomal protein in the 30 S subunit of chloroplast ribosomes and isolation of the cDNA clone encoding its cytoplasmic precursor.
Identification of a plastid-specific ribosomal protein in the 30 S subunit of chloroplast ribosomes and isolation of the cDNA clone encoding its cytoplasmic precursor.
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叶绿体核糖体 30 S 亚基中质体特异性核糖体蛋白的鉴定以及编码其细胞质前体的 cDNA 克隆的分离。
DOI：
10.1016/s0021-9258(19)38228-6
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
A. Subramanian
中科院分区：
文献类型：
--
作者：
C. Johnson;V. Kruft;A. Subramanian
我们描述了叶绿体核糖体蛋白的分离和鉴定及其cDNA的克隆。该蛋白与任何大肠杆菌核糖体蛋白或任何已知蛋白都没有同源性。由于这一新发现，我们建议将其称为PSrp-1，即质体特异性核糖体蛋白。从cDNA序列推断的PSrp-1的前体形式长302个氨基酸残基。成熟的PSrp-1，通过氨基端测序鉴定，是一个236个残基的蛋白质。NH 2-末端66个氨基酸形成将PSrp-1靶向进入叶绿体的转运肽。我们表明，PSrp-1是叶绿体30 S核糖体亚基的蛋白质通过蛋白质印迹和测序后的二维凝胶电泳切下的蛋白质。讨论了PSrp-1可能起源于内共生体理论的有核宿主细胞的进化起源。
We describe the isolation and characterization of a chloroplast ribosomal protein and a clone of its cDNA. This protein has no homology to any Escherichia coli ribosomal protein or to any known proteins. Due to this novel finding we propose it be called PSrp-1, i.e. a plastid-specific ribosomal protein. The precursor form of PSrp-1, deduced from the cDNA sequence, is 302-amino acid residues long. The mature PSrp-1, identified by amino-terminal sequencing, is a protein of 236 residues. The NH2-terminal 66 amino acids form the transit peptide that targets PSrp-1 into the chloroplast. We show that PSrp-1 is a protein of the chloroplast 30 S ribosomal subunit by Western blotting and sequencing the excised protein after two-dimensional gel electrophoresis. The possible evolutionary origin of PSrp-1 from the nucleated host cell of the endosymbiont theory is discussed.